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IL-6

Manufactured by Aviscera Bioscience
Sourced in United States

IL-6 is a quantitative ELISA kit designed for the measurement of human interleukin-6 (IL-6) levels in biological samples. IL-6 is a cytokine involved in various biological processes, including the inflammatory response.

Automatically generated - may contain errors

3 protocols using IL-6

Human TMJ synovial fluid and supernatants, following 5 min centrifugation at 12,500 × g and 4°C, obtained from cultured primary TMJ synovial cells were analysed for the presence of EDPs, IL-1β, IL-6, TNF-α and matrix metalloproteinase-12 (MMP-12) using the appropriate ELISA kit (EDPs, cat. no. SK00806-01; Aviscera Bioscience Inc., Santa Clara, CA, USA; IL-1β, cat. no. DLB50; IL-6, cat. no. D6050; TNF-α, cat. no. DTA00C; all from R&D Systems, Inc., Minneapolis, MN, USA; MMP-12, cat. no. EK0950; Boster Biological Technology, Pleasanton, CA, USA) according to the manufacturer's protocols. Total protein concentrations in the synovial fluid were determined by the dye binding method using an acidic solution of Coomassie Brilliant Blue G-250 dye, according to the manufacturer's protocol (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Values were calculated as pg/mg total protein or ng/mg total protein. Data are presented as the mean ± standard error of the mean.
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Prostate tissues were homogenized in phosphate buffer saline using a motorized tissue homogenizer (Talboys Engineering Corporation, Montrose, USA). The homogenate was centrifuged for 5 mins at 1,000g to remove cell debris and then the supernatant was recentrifuged at 10,000g for 10 mins. Commercially available ELISA kits were used according to the manufacturer protocols to identify prostate tissue levels of TGF-β1 (Aviscera Bioscience, USA), VEGF-A (Abcam, Cambridge, MA) and interleukin-6 (IL-6: Aviscera Bioscience, USA). Protein concentrations were measured by using bicinchoninic acid (BCA) protein assay kit (Sigma, USA) according to the manufacturer’s instructions.
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By using the manufacturer protocol, we have estimated the concentration of various cytokines: tumor necrosis factor-alpha (TNF-α) RayBio-Tech, Inc. (Norcross, GA, USA), IL-6 (interleukin-6), and TGF-β1 (transforming growth factor-beta 1) of Aviscera-Bioscience, Inc. (Santa Clara, CA, USA) in serum.
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