Shoot fresh (FW) and dry (DW) weight were measured, and relative water content calculated as RWC = (FW-DW)/FW. Before harvesting, leaf chlorophyll content was measured as a SPAD index using a Minolta Chlorophyll Meter SPAD-502 (Konica Minolta, Osaka, Japan) on the third true leaf, at a position about one quarter of the length of the leaf from the leaf tip. Stomatal conductance (Gs) was also measured on the same leaf using a Decagon leaf porometer (Decagon Devices Inc., WA, Australia), under constant light conditions (artificial light of 150 μmol m−2 s-1). The number of necrotic leaves was also counted at harvest.
Decagon leaf porometer
Manufactured by METER Group
Sourced in Australia, Japan
The Decagon leaf porometer is a lab equipment device that measures the stomatal conductance of leaves. It provides a quantitative assessment of the rate at which water vapor and other gases diffuse through the stomata, which are the pores on the surface of a leaf.
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Lab products found in correlation
3 protocols using decagon leaf porometer
1
Leaf Water and Chlorophyll Analysis
2
Evaluating Plant Physiological Parameters
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Chlorophyll content and stomatal conductance were measured on six randomly selected youngest fully expanded leaves of each treatment. All measurements were taken on a sunny day between 11:00 am and 1:00 pm, to minimize the diurnal influences. The chlorophyll content was measured using a Minolta Chlorophyll Meter SPAD-502 (Konica Minolta, Osaka, Japan); a Decagon leaf porometer (Decagon Devices Inc., Pullman, WA, USA) was used for the stomatal conductance measurements. Plants were then harvested, and their fresh weight was measured. Plants were then dried at 65 °C in a drier (Unitherm, Birmingham, UK) and their dry weight was recorded.
3
Assessing Leaf Responses to Salinity
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The number of chlorotic and necrotic leaves per plant was recorded after 5 weeks of salt treatment. Leave which were partially yellow leaves were deemed as chlorotic leaves. Completely dead and dried leaves were deemed as necrotic. Relative leaf chlorophyll content was measured using a SPAD-502 Chlorophyll Meter (Konica Minolta). Measurements were taken on the third true leaf, at a position about one-quarter of the length of the leaf from the leaf tip. The same leaf/position was used to measure stomatal conductance (g s ) from control and salt treated plants using a Decagon leaf porometer (Decagon Devices Inc.), under constant light conditions (artificial light of 150 mmol m À2 s -1 ) between 1100 and 1500 hours.
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