A complete list of plasmids and the sequences of oligonucleotides used in this work can be found in Supplementary Tables 1–2 . gBlock® gene fragments and natural oligonucleotides (with standard purification and desalting) were purchased from IDT (San Diego, California, USA). Sequencing was performed by Genewiz (San Diego, California, USA). Plasmids were isolated using commercial miniprep kits (QIAprep, Qiagen or ZR Plasmid Miniprep Classic, Zymo Research). pEVOL-pAzF14 (link) was kindly provided by Peter Schultz (The Scripps Research Institute, USA).
Nucleosides of dNaM , dTPT3 , NaM , TPT3 , d5SICS and dMMO2bio were synthesized (WuXi AppTec, Shanghai, China) and triphosphorylated (TriLink BioTechnologies LLC, San Diego, USA and MyChem LLC, San Diego, USA) commercially. All unnatural oligonucleotides were synthesized by Biosearch Technologies (Petaluma, California, USA) with purification by reverse phase cartridge.
Nucleosides of d
