Huvecs
HUVECs (Human Umbilical Vein Endothelial Cells) are primary cells derived from the human umbilical vein. They are used in cell culture research and serve as a model for studying endothelial cell biology and function.
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23 protocols using huvecs
Cultivation of Human Umbilical Vein Endothelial Cells
HUVEC Culture in Normal and High Glucose
Isolation and Culture of DPSCs and HUVECs
Isolation and Characterization of Human Pancreatic Stellate Cells
Culturing HCC and Endothelial Cells
IU/mL penicillin G and 100 mg/mL streptomycin sulfate (Sigma-Aldrich, St. Louis, MO, USA) in a humidified atmosphere of 5% CO2 at 37°C.
TECs and normal ECs (NECs) were isolated from surgical HCC specimens and the surrounding normal liver tissues, as previously described (29 (link)). Primary human umbilical vein endothelial cells (HUVECs; AllCells, Shanghai, China) were cultured in endothelial cell growth medium-2 (Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum and were used within 2-10 passages.
Conditioned media (CM) for ECs was generated according to our previous study (30 (link)).
Cell Culture and Transfection Protocols
Silencing SVIL in Liver Cancer Cells
Cells were transfected with SVIL Stealth siRNA: E4 double stranded (ds)RNA, E5 dsRNA, E11 dsRNA and negative control dsRNA (all Invitrogen; Thermo Fisher Scientific, Inc.; all 40 nM) using Lipofectamine® RNAi-MAX (Invitrogen; Thermo Fisher Scientific, Inc.). The dsRNA targeting sequences were as follows: E4 dsRNA, 5′-CUCACUUUGAAUGUAGAGAACCAUC-3′; E5 dsRNA, 5′-UUCUGCUGAAGUUAUAGGUUGGGUU-3′; E11 dsRNA, 5′-AGCAUAUUUAGAUUCCUUAUGGCUG-3′.
HepG2 cells were treated with or without 50 µg/l recombinant human VEGF (Novus Biologicals, LLC) at the indicated time-points.
Endothelial Cell Culture and PM2.5 Treatment
PM2.5 was resuspended in PBS and the solutions were stored at −20°C until use. ECs were treated with various concentrations of PM2.5 to select the optimal concentration and time.
Comparative Cell Proliferation and Migration Assays
HUVEC Angiogenesis Model with bFGF
To simulate the in vitro CRNV models, HUVECs were treated with 5, 10 or 20 ng/ml essential fibroblast growth factor (b-FGF) for 24 h.
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