Dichloromethane

PLLA (molecular weight = 160 kDa) and PDLLA (molecular weight = 150 kDa) nanofiber scaffolds were prepared as follows. PLLA and PDLLA were dissolved in a mixture of dichloromethane (Amresco, California, USA) and N,N-dimethylformamide (Amresco, California, USA) at a ratio of 7:3, achieving a concentration of 8%–10%. The mixture was heated to 37°C for 3–4 h in a water bath until materials were completely dissolved. Next, using a 10 mL syringe connected to an injection pump (WZ-50C2, China) set to 18 kV (DWP503–1AC, China), electrospinning was conducted at 40 μL/min for approximately 4 h at room temperature (Zhang et al., 2024 (link)).

We extracted the volatile organic substances from a fresh wood sample and a fruit body of P. pouzarii from the same log (TA19); both were not dried and kept at -18 °C prior to extraction. Corresponding samples were dried to determine the moisture content and to calculate the dry matter content. Both sample types were cut into small pieces (< 3 mm), weighed (1.7 g wood sample, 0.3 g fruit body sample) and transferred into reaction tubes. The extraction solvent dichloromethane (VWR Chemicals, Delaware, USA) for gas chromatography-mass spectrometry (GC-MS) or acetone (Merck, Darmstadt, Germany) for high performance liquid chromatography-mass spectrometry (LC-MS)) was added at the ratio of 1:3.25 and 1:6.5, respectively. Samples were mechanically stirred for 6 h and then subjected to an ultrasonic treatment for 30 min (Sonorex, Bandelin, Berlin, Germany). Subsequently, reaction tubes were centrifuged (8,000 rpm, 20 min) and liquid extracts removed. For details and a full list of authentic standards, please compare supplement S1.

Experimental Materials: Outdoor pond‐cultivated C. reevesii (OP) and greenhouse‐cultivated C. reevesii (GH), both sourced from Jiangxi Jinguiwang Industrial Co. Ltd. All C. reevesii used in the experiment were female and 4 years old. The culture conditions of OP before the experiment were as follows: outdoor ponds, a culture density of 8/m², feeding twice a day, and 2% of body weight. Before the experiment, GH was reared in an indoor greenhouse at a constant temperature of 26°C ± 2°C, with a culture density of 8/m², feeding three times a day and 2% of body weight. The average weight of the OP was 716.66 ± 45.6 g, while the GH averaged 658.75 ± 38.85 g. C. reevesii was transported to the laboratory at the Yangtze River Fisheries Research Institute of the Chinese Academy of Fishery Sciences and placed in cultivation tanks measuring 2 × 1 × 0.3 m with a water depth of 3 cm. The stocking density is about 8/m². The water was changed daily, and no food was provided as the C. reevesii underwent a temporary rearing experiment (water temperature maintained at 25°C ± 2°C). Samples were taken on days 0 (control), 10, 20, 30, 40, 50, and 60 of temporary rearing. Four C. reevesii from OP and GH was sampled each time. The skin was removed, and the limb muscles were harvested, immediately frozen in liquid nitrogen for 3 min, and then thoroughly ground into a powder using a grinder. The resulting frozen C. reevesii meat powder was stored at −80°C for subsequent volatile compound analysis.
Chemicals: Dichloromethane (HPLC, 99.9%) was purchased from Avantor Performance Materials. Sodium chloride (HPLC, ≥ 99.5%), hexanol (≥ 99.5%), heptanal (≥ 98%), (E)‐2‐nonenal (≥ 95%), indole (≥ 99.5%), (E,E)‐2,4‐decadienal (≥ 90%), pentadecanal (95%), tridecanal (95.5%), hexadecanal (98%), hexanoic acid (≥ 99.5%), and nonanoic acid (≥ 99.5%) were procured from Shanghai Macklin Biochemical Co. Ltd. Decanal (≥ 98%) and nonanal (≥ 98%) were acquired from Shanghai Yuan Ye Biological Technology Co. Ltd. Hexanol (100 μg/mL) and dodecanol (100 μg/mL) were bought from Dr. Ehrenstorfer. (E)‐2‐decenal (100 μg/mL), octanal (1000 μg/mL), heptanol (100 μg/mL), tetradecanol (1000 μg/mL), γ‐nonanolactone (1000 μg/mL), and naphthalene (1000 μg/mL) were obtained from Tianjin Alta Scientific Co. Ltd. Octanol (≥ 99.5%), dodecanal (≥ 99.5%), 1‐octen‐3‐ol (≥ 99.9%), and 2‐pentylfuran (≥ 99.2%) were obtained from Beijing Tanmo Quality Inspection Technology Co. Ltd.