The recombinant EGFR protein (extracellular part aa 1–645 and intracellular part aa 668–1210) was from Sinobiologicals, China. The EGFR protein was bound to ELISA plates (1 μg/ml) for overnight at 4 °C. 100 μl serum of lung cancer patients were added and incubated for 1 h at room temperature. The plates were washed for 3 times by washing buffer (PBS with 0.05% Tween-20), and incubated with HRP labeled goat anti-human IgG for 1 h, followed by colorimetric detection. PBS 1% BSA was used as blank for determining the cutoff value.
Adx egfr mutations detection kit
The ADx EGFR Mutations Detection Kit is a laboratory equipment product designed to detect mutations in the epidermal growth factor receptor (EGFR) gene. The kit utilizes a molecular biology-based approach to identify specific genetic alterations associated with various types of cancer.
Lab products found in correlation
14 protocols using adx egfr mutations detection kit
EGFR Autoantibody Detection in Lung Cancer
The recombinant EGFR protein (extracellular part aa 1–645 and intracellular part aa 668–1210) was from Sinobiologicals, China. The EGFR protein was bound to ELISA plates (1 μg/ml) for overnight at 4 °C. 100 μl serum of lung cancer patients were added and incubated for 1 h at room temperature. The plates were washed for 3 times by washing buffer (PBS with 0.05% Tween-20), and incubated with HRP labeled goat anti-human IgG for 1 h, followed by colorimetric detection. PBS 1% BSA was used as blank for determining the cutoff value.
EGFR Mutation Detection in Tumors
EGFR Mutation Detection in Tissue Specimens
Molecular Biomarker Detection in Lung Cancer
EGFR Mutation Detection Protocol
Screening for EGFR Mutations in MPE
EGFR Mutation Detection in NSCLC Tissues
A Human EGFR Gene Mutation (exons 18–21) Fluorescence Polymerase Chain Reaction (PCR) Diagnostic Kit (Amoy Diagnostics), which was based on ARMS technology, was used to analyze the DNA from the tissue samples. An ADx EGFR Mutations Detection Kit (Amoy Diagnostics) has received China Food and Drug Administration (CFDA) approval for clinical usage since 2010. We defined a cut-off of 2% tumor cell content as a sample quality check according to the minimum requirement of ARMS technology (approximately 1% analytical sensitivity). Samples below this threshold were rejected.
EGFR and KRAS Mutation Detection
EGFR Mutation Analysis in NSCLC
EGFR Mutation Detection in PC-9-GR Cells
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