ELISA
ELISA (Enzyme-Linked Immunosorbent Assay) is a biochemical technique used to detect and quantify specific substances, typically proteins, antibodies, or other biomolecules, in a liquid sample. The core function of ELISA is to measure the presence and concentration of a target analyte through the use of antibodies and color-change or fluorescent reactions.
Lab products found in correlation
54 protocols using ELISA
Norepinephrine Levels in Hippocampus and Plasma After Conditioning
Plasma LBP, PAI-1, and Intestinal Arginase
Saliva sIgA Quantification in Newborns
HBV Infection Inhibition Assay
Alternatively, PXB cells were treated with preS1 peptide, anti-OVA MAb, or N6HB426-20 MAb for 1 h and washed with PBS. Cells were infected with HBV (genotype C or genotype D derived from HBV-infected patients) at a concentration of 10 genome equivalents per cell in the presence of 4% PEG 8000 and 2% DMSO, along with preS1 peptide or each antibody. At 24 h postinfection, cells were washed with PBS. HBV-infected cells were cultured in fresh medium containing 2% DMSO. At day 7 postinfection, the level of HBsAg was determined by a chemiluminescent enzyme immunoassay (Lumipulse f, Fujirebio, Japan).
HBeAg Quantification by ELISA
Plasma Cytokine Profiling Post-MCAo
Triamcinolone-loaded CD90-targeted Nanoparticles
The structure micro humid of the CD90@NP was observed using the HT7800 transmission electron microscope (Hitachi Ltd, Tokyo, Japan). The ζ potential (mA) and particle size (nm) of the CD90@MV, NP, RNP, and CD90@NP were detected by a Malvern Zetasizer Nano ZS90 nanoparticle size system (Malvern, UK). T-CD90@NP was collected via centrifugation at 12,000×g for 45 min and dissolved in dimethyl sulfoxide to determine the DLC and EE of TA. The TA content in T-CD90@NP was tested by ELISA (Abnova, Taipei, Taiwan). The following formula was used to calculate the DLC and EE:
Cytokine, Intestinal Barrier, and Bacterial Translocation Analysis
To study intestinal barrier damage, the concentration of I-FABP and zonulin in the serum were measured by enzyme-linked immunosorbent assay (ELISA). I-FABP was purchased from Hycult Biotech (Hycult Biotech, PA, USA), and zonulin was purchased from R&D Systems (R&D Systems, MN, USA). #e tests were carried out according to the protocols provided in the kits. #e plate was read in an iMark Microplate Reader at 450 nm with Microplate Manager So(ware (Termo Fisher Scientific, MA, USA).
To study bacterial translocation, the concentration of LBP in the serum was measured by ELISA (Abnova, Taipei, Taiwan). We performed 1:800 dilutions of the samples from the MDD patients and HCs. The test was carried out according to the protocol provided in the kit. The plate was read in an iMark Microplate Reader at 450 nm with Microplate Manager Software (Thermo Fisher Scientific).
Plasma PSA ELISA Quantification
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