Ketamine hydrochloride
Ketamine hydrochloride is a chemical compound commonly used as a general anesthetic and analgesic in medical and veterinary settings. It is a white, crystalline powder that is soluble in water and alcohol. The primary function of ketamine hydrochloride is to induce a dissociative anesthetic state, resulting in pain relief, sedation, and amnesia.
Lab products found in correlation
9 protocols using ketamine hydrochloride
Serum Corticosterone Measurement Protocol
Isolation and Culture of Rat Endometrial Cells
of the eighth week (56th day) of the study and 4 days after the observation of
sperm in the vaginal smears, rats in all groups were sacrificed by intraperitoneal
injection of ketamine hydrochloride (50 mg/kg, Alfasan, Woerden, Netherlands) and xylazine
hydrochloride (Alfasan, Woerden, Netherlands, 7 mg/ kg,
dissected and the endometrium was removed in a sterile condition. The endometrial samples
were washed with Hanks’ balanced salt solution (Sigma-Aldrich, St. Louis, MO, USA), then
chopped into several fragments and kept in the sterile micro tubes. The endometrium of one
uterine horn was used directly for molecular experiments, and another was used for cell
isolation and culture, followed by molecular experiments on day 7.
The isolated cells in the all groups were maintained in
DMEM F-12 (1:1, Sigma-Aldrich, St. Louis, MO, USA)
containing fetal calf serum (FCS, 10%, Sigma-Aldrich,
St. Louis, MO, USA), penicillin (Aldrich-St. Louis, MO, USA) and streptomycin (Sigma-Aldrich, St. Louis, MO,
USA). The cells were then transferred to special culture
flasks. Initial incubation was carried out for 2 hours at
37˚C with 5% carbon dioxide concentration. Then, every
three days, the cell culture medium changed.
Sciatic Nerve Transection and Repair
Rabbit Gingival Graft Healing and Geometry
Histological analyses were carried out 7 and 28 days after FGG. At each time interval of analysis (days 7 and 28), 10 animals, 5 per group, were killed by an overdose of ketamine and xylazine. GT and KTW were evaluated at baseline and 7 and 28 days after surgery by an endodontic reamer with a silicon stop.15 (link) Furthermore, the wound healing index was assessed 7 and 28 days after surgery.
The wound healing index was graded as below: Grade 1: healing without any major events, with no edema, gingival erythema, pus discharge, or opening of the wound edges. Grade 2: healing without any major events; healing with a small amount of edema or gingival erythema or opening of the wound edges, but without any discharge of pus. Grade 3: poor wound healing with marked gingival edema, erythema, and opening of the wound edges or any pus discharge.16 (link) All the surgical procedures were performed by an experienced surgeon (A.M.), and all the measurements were made by another blinded operator (S.Sh.).
Anesthetic Cocktail Preparation
Primate Hypertension Evaluation Protocol
Deoxycholate and BBR Extraction Protocol
Stabilization and Preservation of Rice Bran
The fresh rice bran variety IR 64 (Oryza sativa L.) was obtained from Bogor, West Java, Indonesia. The fresh rice bran samples in a drying tray were inserted into an oven (Memmert, Germany), which was set at 110 • C for 15 min. After that, the rice bran sample was cooled in a container for 30 min to reach room temperature. The stabilized rice bran was then put in clear plastic and stored at room temperature.
Canine Dental Pulp Extraction Protocol
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