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Corticosterone enzyme immunoassay kit

Manufactured by Arbor Assays
Sourced in United States, France

The Corticosterone Enzyme Immunoassay Kit is a laboratory tool used to quantify corticosterone levels in various sample types. It employs an enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the concentration of this hormone.

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22 protocols using corticosterone enzyme immunoassay kit

1

Quantifying Stress Hormones in Rats

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Caval plasma CRH, ACTH, and corticosterone concentrations were assayed using a Corticotropin Releasing Factor Extraction Free EIA Kit (Phoenix Pharmaceuticals, Burlingame, CA), ACTH Extraction Free EIA Kit (Phoenix Pharmaceuticals), and a Corticosterone Enzyme Immunoassay Kit (Arbor Assays, Ann Arbor, MI), respectively. Tail vein plasma corticosterone levels were also assayed using a Corticosterone Enzyme Immunoassay Kit. The intra-assay and inter-assay coefficients of variation were <10 and 15%, respectively. The area under the curve (AUC) of the plasma corticosterone levels during the ACTH stimulation test was calculated.
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2

Blood and Brain Tissue Collection for Metabolic Analysis

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Blood samples were collected from the retroorbital vein under isoflurane anesthesia and immediately transferred to tubes containing ethylenediaminetetraacetic acid (EDTA; 10 μl of 0.2 M EDTA/tube) and aprotinin (0.1 mg/tube, Merck KGaA). The blood samples were centrifuged 3000×g for 5 min at 4 °C, and the plasma was separated and stored at −80 °C until assayed. After blood collection, the mice were killed by decapitation. The brain was rapidly removed from the skull and placed on an ice-cooled paraffin plate for dissection of the hippocampus as previously described (Nakao et al., 1986 (link)). The hippocampus was immediately frozen in liquid nitrogen and stored at −80 °C until analyzed. Glucose (Glucose C2; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), insulin (Morinaga Ultra Sensitive Mouse/Rat Insulin ELISA Kit; Morinaga Institute of Biological Science, Yokohama, Japan), corticosterone (Corticosterone Enzyme Immunoassay Kit; Arbor Assays, Ann Arbor, MI, USA) and IGF-1 (Mouse/Rat IGF-1 Quantikine ELISA Kit; R&D Systems, Inc., Minneapolis, MN, USA) were measured using commercially available kits.
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3

Restraint Stress Effects on Neurotransmitters

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Immediately after the mice subjected 1, 7, and 21 cycles of restraint stress, approximately 1.00 ml of blood was drawn from the retro-orbital plexus. The blood samples were allowed to clot at room temperature for 1 h, afterward, centrifuged at 5000 rpm for 10 min and serum stored at −80 °C for subsequent. Serum norepinephrine and cortcosterone were measured with mouse norepinephrine ELISA kit (Westang, Shanghai, China) and corticosterone Enzyme Immunoassay kit (Arbor Assays, Michigan, USA) following the manufacturer’s instructions, respectively.
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4

Corticosterone ELISA Quantification Protocol

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Serum corticosterone concentration was measured in duplicate using a commercial ELISA kit (Corticosterone Enzyme Immunoassay Kit, Arbor Assays, Euromedex, Souffelweyersheim, France) according to the manufacturer’s instructions.
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5

Plasma Hormone and Amino Acid Analysis

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Blood was collected from the tip of the tails, and blood glucose levels were measured with an Antsense Duo Small Electrode Glucose Analyzer (Horiba, Kyoto, Japan). Blood samples were centrifuged (13,500 rpm, 10 min, 4 °C) twice, and the collected plasma samples were stored at −80 °C until the biochemical analyses. Plasma hormone levels were measured using the following assays: insulin, Mouse/Rat Insulin ELISA Kit (Morinaga Institute of Biological Science, Kanagawa, Japan); FGF21, Mouse and Rat FGF21 ELISA Kit (BioVendor Inc., Brno, Czech Republic); GCG, Glucagon ELISA Kit (Mercodia, Uppsala, Sweden); active GLP-1, GLP-1 Active Form Assay Kit (IBL, Gunma, Japan); GIP, GIP (total) ELISA kit (Millipore, Billerica, MA, USA); and corticosterone, Corticosterone Enzyme Immunoassay Kit (Arbor Assays, Ann Arbor, MI, USA). Plasma amino acid concentrations were measured by standard assays at SRL, Tokyo, Japan [21 (link)].
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6

Plasma Corticosterone Extraction and Quantification

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Plasma was extracted from blood samples collected from the heart. All blood samples were collected between 2 and 4 p.m. Blood samples were centrifuged at 3,000 rpm for 5 min at 4°C and extracted plasma was stored at −30°C until assayed for corticosterone using a corticosterone enzyme immunoassay kit (Arbor Assays, Ann Arbor, MI) in accordance with the manufacturer’s instructions.
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7

Direct Corticosterone Quantification

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Corticosterone levels in serum samples were measured without any extraction procedure using the Corticosterone Enzyme Immunoassay kit (Arbor Assays, Ann Arbor, MI, United States). Concentrations were calculated using a 4-parameter curve and expressed in ng/ml.
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8

Corticosterone Quantification in Mouse Plasma

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Mice were euthanized by cervical dislocation immediately followed by decapitation. Trunk blood was collected in an EDTA-coated tube. Plasma was separated by centrifugation at 600 g for 15 min at 4 °C and stored at −80 °C. Corticosterone levels were measured in duplicates using the Corticosterone Enzyme Immunoassay Kit (Arbor Assays, Ann Arbor, MI) according to the manufacturer’s instructions.
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9

Serum Corticosterone Quantification

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Corticosterone was quantified in serum samples without any extraction procedure using the Corticosterone Enzyme Immunoassay kit (ArborAssays, Ann Arbor, MI, USA). Samples were analyzed in duplicate. Absorbance at 405 nm was measured and concentrations, calculated from a standard curve established using calibrators, were expressed as ng/mL.
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10

Steroid Hormone Quantification in Animals

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Circulating concentrations of corticosterone (CORT), testosterone (T), and luteinizing hormone (LH) were measured in all animals using an ELISA assay kit according to the manufacturer’s instructions (Corticosterone Enzyme Immunoassay Kit, cat #K014-H1, lot #16CS080d; Arbor Assays, Ann Arbor, MI; Testosterone Enzyme Immunoassay Kit, cat #K032-H1, lot #17T051b; Arbor Assays; LH ELISA Kit, cat #ENZ-KIT107-0001, lot #02061708A; Enzo Life Sciences, Farmingdale, NY). For all assays, samples that fell below the limit of detection were included in the analysis with a value equal to that of the detection limit, as indicated by the dashed line in each graph.
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