Protein was extracted from frozen mouse kidneys and immunoblot analysis was performed as previously described 10 using the following primary antibodies: anti-KIM-1 (AF1817, RD Systems), anti-SOX-9 (NBP1–8551, Novus Bio), anti-SPTLC2 (51012–2-AP, Proteintech), anti-ABCD3 (PA1–650, Invitrogen), anti-ACOX1 (ab184032, Abcam), anti-EHHADH (GTX81126, Genetex), anti-HSD17B4 (15116–1-AP, Proteintech), anti-ACAA1 (12319–2-AP, Proteintech), anti-SCPx (HPA027135, Atlas Antibodies), anti-CROT (NBP1–85501, Novus Bio), anti-CPT2 (26555–1-AP, Proteintech), anti-MCAD (55210–1-AP, Proteintech), anti-α-tubulin (32–2500, Thermo Fisher), anti-citrate synthase (GTX628143, Genetex), and anti-AMACR 16 (link).
Gtx628143
Manufactured by GeneTex
The GTX628143 is a laboratory centrifuge that is designed for general-purpose applications. It features a compact and durable construction, making it suitable for use in various laboratory settings. The centrifuge can accommodate a range of sample volumes and tube sizes, allowing researchers to efficiently separate and process their samples.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (4 mentions)
Ab184032, by Abcam (3 mentions)
Anti α tubulin, by Thermo Fisher Scientific (3 mentions)
Gtx81126, by GeneTex (3 mentions)
Protease and phosphatase inhibitor, by Thermo Fisher Scientific (2 mentions)
Ab128565, by Abcam (2 mentions)
Irdye 800cw, by LI COR (2 mentions)
Irdye 680rd, by LI COR (2 mentions)
Amab90618, by Atlas Antibodies (2 mentions)
Bolt 4 12 bis tris plus gel, by Thermo Fisher Scientific (2 mentions)
Af1817, by R&D Systems (1 mentions)
Nbp1 85501, by Novus Biologicals (1 mentions)
3 protocols using gtx628143
1
Protein Extraction and Immunoblot Analysis
2
Western Blot Analysis of Metabolic Enzymes
3
Quantitative Western Blot Analysis of Lipid Metabolism Enzymes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells and tissues were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (Thermo Fisher Scientific), followed by sonication and centrifugation (10 min at 12,000 rpm at 4°C). Protein concentration was determined by the BCA method. Proteins were separated on Bolt TM 4-12% Bis-Tris Plus gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092, LI-COR) and detected using the following primary antibodies: anti-ACOX1 (ab184032, Abcam), anti-ACAA1 (12319-2-AP, Proteintech), anti-EHHADH (GTX81126, Genetex), anti-HSD17B4 (ab128565, Abcam), anti-HMGCR (AMAb90618, Atlas Antibodies), anti-citrate synthase (GTX628143, Genetex), and anti-α-tubulin (32-2500, Thermo Fisher). Anti-MVK and anti-PMVK antibodies were a gift from Dr. Hans Waterham (AMC, Amsterdam) (Hogenboom et al. 2004b,a) . Proteins were visualized with goat anti-mouse and goat anti-rabbit secondary antibodies IRDye 800CW and IRDye 680RD (LI-COR, 926-32 210, 926-68 070, 926-32 211, 926-68 071) . Band intensities were quantified using the Fiji distribution of ImageJ 1.x (Schindelin et al. 2012) .
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