Plasmid Mini Kit
The Plasmid Mini Kit is a laboratory product designed for the rapid and efficient purification of plasmid DNA from bacterial cultures. It utilizes a spin column-based method to isolate high-quality plasmid DNA suitable for various downstream applications.
Lab products found in correlation
29 protocols using Plasmid Mini Kit
Engineered Y. lipolytica for Efficient Xylose Utilization
Plasmid Isolation and DNA Manipulation
Cloning and Transformation in Yarrowia lipolytica
For transformation of Yarrowia lipolytica only strains with auxotrophy for uracil were used.
Transformation was performed according to the lithium acetate method24 (link) and transformants were plated out on selective media without uracil. They were analyzed for proper integration by gDNA extraction and PCR amplification with two primer pairs. Genomic DNA (gDNA) was extracted from Y. lipolytica using the Genomic Mini AX Yeast Spin kit (A&A Biotechnology, Poland).
Yeast Genomic DNA Library Preparation
Yeast genomic DNA library in the pFL44L plasmid was used (Lacroute, Gif-sur-Yvette).
Plasmid preparation, E. coli transformation and agarose gel electrophoresis were carried out as described in Sambrook and Russel [74 ].
Isolation of plasmid DNA was performed using standard alkaline lysis method or with Plasmid Mini Kit (A&A Biotechnology), according to the product manual.
Cloning and Characterization of Mutant c23o Gene
Yeast Transformation and Plasmid Isolation
Genetic Manipulation Protocols for Paracoccus spp.
Recombinant C. albicans Enolase Expression
Plasmid Isolation from Bacterial Cultures
Integrating Heterologous Genes into Yarrowia lipolytica
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