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Colo205

Manufactured by Thermo Fisher Scientific
Sourced in United States, China
About the product

The Colo205 is a laboratory instrument designed for the analysis and measurement of various samples. It utilizes advanced techniques to provide accurate and reliable data. The core function of the Colo205 is to perform analytical tasks, but its specific intended use is not included in this factual description.

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The spelling variants listed below correspond to different ways the product may be referred to in scientific literature.
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63 protocols using «colo205»

1

Propagation of Colorectal Cancer Cell Lines

2025
The Chinese Academy of Sciences’ Cell Bank was utilized to obtain the CRC cell lines LoVo and Colo-205. The cell lines CT-26 and MC-38 were from Shanghai Zhongqiao Xinzhou Biotechnology Co., Ltd. The LoVo cells were grown in Gibco’s F-12 Nutrient Mix medium (#11765054), whilst the Colo-205, MC-38, and CT-26 cells were kept in Gibco’s RPMI-1640 medium (#11875093). In order to enhance the media, 10% fetal bovine serum (FBS) from Biological Industries in Northern Kibbutz Beit Haemek, Israel, and 1% penicillin/streptomycin from Gibco (#10378016) were added. The cell cultures were kept in an incubator with 5% CO2 at 37 °C.
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2

Propagation of Colorectal Cancer Cell Lines

2025
The Chinese Academy of Sciences’ Cell Bank was utilized to obtain the CRC cell lines LoVo and Colo-205. The cell lines CT-26 and MC-38 were from Shanghai Zhongqiao Xinzhou Biotechnology Co., Ltd. The LoVo cells were grown in Gibco’s F-12 Nutrient Mix medium (#11765054), whilst the Colo-205, MC-38, and CT-26 cells were kept in Gibco’s RPMI-1640 medium (#11875093). In order to enhance the media, 10% fetal bovine serum (FBS) from Biological Industries in Northern Kibbutz Beit Haemek, Israel, and 1% penicillin/streptomycin from Gibco (#10378016) were added. The cell cultures were kept in an incubator with 5% CO2 at 37 °C.
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3

Culturing Colo-205 and KHYG-1 Cells

2024
Colo-205 human colon cancer cells were purchased from the Korea Cell Line Bank (KCLB Korea), and KHYG-1 human natural killer cells were purchased from AcceGen Biotech (ABC-TC0506, USA). Colo-205 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin/streptomycin at 37°C in a humidi ed CO 2 incubator with 5% CO 2 .
KHYG-1 cells were cultured under identical conditions as Colo-205, supplemented with an additional 100 U/mL of recombinant human interleukin-2 (Peprotech, 200-02). Both cell types were seeded in a 90 × 20 mm cell culture dish (SPL, 20101). The medium was changed every 3-4 days.
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4

Cell Line Culture and Authentication

2024
Cell lines used in this study were purchased
from the American Type Culture Collection via LGC Standards. Human
normal lung fibroblasts (MRC-5), alveolar basal epithelial cell adenocarcinoma
(A549), colorectal adenocarcinoma (Colo205), hepatocellular carcinoma
(HepG2), breast adenocarcinoma (MCF7), and colorectal adenocarcinoma
(SW620) and its MDR variants55 (link) were cultured
in standard conditions (37 °C, 5% CO2, 100% relative
humidity) in high glucose DMEM medium supplemented with GlutaMax,
HEPES (ThermoFisher Scientific) and 10% fetal bovine serum (EURx,
Poland). All cell lines were tested for Mycoplasma contamination using a MycoProbe mycoplasma detection kit (R&D
System).
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5

CRC Cell Line Genetic Manipulation

2024
Human embryonic kidney cell line (HEK-293T), CRC cell lines (HCT15, HCT8, DLD1, RKO, SW480, HCT116, SW620, LoVo, HT29, Caco2, COLO205, and SW1116), and normal colon epithelial cell line (NCM460) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). RPMI-1640 medium (Gibco, USA) was used to culture HCT15, HCT8, and DLD1 cells, while DMEM medium (Gibco, USA) was applied to cultivate HCT116, RKO, SW480, SW620, LoVo, HT29, Caco2, COLO205, SW1116, NCM460, and HEK-293T. Both media contain 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. PCR method was used to routinely check for mycoplasma contamination.
To knock down HIF1A-AS2 in CRC cells, short hairpin RNAs (shRNAs) were cloned into the pGLV3/H1/GFP/puro vector (sh-HIF1A-AS2#1/sh1 and sh-HIF1A-AS2#2/sh2) (Shanghai Fanxu Technology). Full-length HIF1A-AS2 was cloned into pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid (ex-HIF1A-AS2/HIF1A-AS2) to overexpress HIF1A-AS2. HEK-293T cells were transfected with the knockdown/overexpressed plasmids, psPAX2, and pMD2.G, using a transfection reagent. After 48 h, the culture medium of HEK-293T cells (containing lentivirus particles encapsulating knockdown/overexpression constructs) was harvested, filtered using a percolator whose bore diameter is 0.45 μm (Millipore, USA), and applied to transfect CRC cells to knock down or overexpress HIF1A-AS2. Stable cell lines were selected using a culture medium containing 5 µg/mL puromycin. Short hairpin RNAs against FOXC1 (sh-FOXC1) and relevant overexpression plasmids (ex-FOXC1), were also designed. SP1, FOXP3, E2F1, wild-type and mutant HIF1A-AS2 were cloned into pcDNA3.1 vector. siRNAs against FOXP3, SP1, E2F1, and the mimics or inhibitors of miR-141-3p were transfected into CRC cell line to change the expression of relevant genes. Supplementary Table 1 summarized the sequences of shRNAs and siRNAs, and Supplementary Table 2 summarized the sequences of the mimics and inhibitors.
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