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Contour Plus One

Manufactured by Ascensia Diabetes Care
Sourced in Switzerland

The Contour Plus One is a blood glucose monitoring system designed for people with diabetes. It is a compact and portable device that allows users to measure their blood glucose levels quickly and easily. The Contour Plus One provides accurate and reliable results, helping individuals to effectively manage their condition.

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6 protocols using Contour Plus One

Five-week old male mice (C57/B6 strain) were purchased from “Andreevka” animal husbandry facility (Moscow region, Russia). After 5 days of acclimation, mice were matched by body weight (17–23 g), fasting blood glucose (FBG) level (3.9–6.9 mM) and randomized to either low fat diet (LFD) or high fat diet (HFD) group (n = 14–16 per group). During the next 10 weeks animals were kept under specific pathogen-free conditions with chow and water ad libitum and regular 12:12 light–dark cycle. Both low-fat diet (LFD) (#D12450H) and high-fat diet (HFD) (#D12451) chows were purchased from “Research Diets”, USA. During the experiment, the body weight, FBG and food consumption were measured every 2 weeks. For the FBG measurements, we used a Contour Plus One glucometer (Ascensia Diabetes Care, Basel, Switzerland). At 10 weeks, all mice underwent surgical modeling of hindlimb ischemia. Experimental protocols including surgical manipulations and euthanasia were designed in accordance to Institute and National regulations. All animal experiments were carried out in compliance with internal “Rules for conducting work using experimental animals” and were approved by the Ethical Board of the National Medical Research Center of Cardiology (permit # 16-10-00).
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Standardised glucometers (Contour Plus One, Ascensia Diabetes Care, 600 North Bridge Road) with blue-tooth connectivity were provided to every participant together with an ample supply of glucose strips for use for SMBG throughout the study period, including the entire Ramadan month. Participants were advised to perform SMBG at least 4 times per day and more frequently during Ramadan fasting (pre-sahur, 2-hour post-sahur, pre-iftar, 2-hour post iftar, or when symptomatic). SMBG readings were recorded by participants into individual diabetes logbooks with other relevant details such as amount and type of food, physical activities, and insulin doses. During Ramadan, the participants could discontinue fasting, if they experience any symptoms of being unwell, hypoglycaemia, severe hyperglycaemia, or sudden change of decision for any personal reasons.
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Diabetes was induced in 6-week-old females (Hif1aloxP/loxP) by two intraperitoneal injections of 100 mg/kg body weight of streptozotocin (STZ, S0130, Sigma-Aldrich, Dorset, UK) within a 1-week interval, as described (37 (link), 48 (link)). The level of blood glucose was checked in a drop of blood from the tail vessel using a glucometer (CONTOUR plus ONE, Ascensia Diabetes Care, Basel, Switzerland). Mice with a level of glucose maintained above 13.9 mmol/l in blood were classified as diabetic. Diabetic females were mated with males, and the next morning, the vaginal plug was checked. Maternal blood glucose levels at the time of embryo collection are shown in Supplementary Figure 1. Embryos collected from diabetic pregnancies were labeled as "diabetic" or "DIA", and from non-diabetic pregnancies were labeled as "non-diabetic" or "non-DIA".
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Adult male C57BL/6 mice (10 weeks old) were purchased from “Andreevka” animal husbandry facility, Russia. Mice were kept under standard pathogen-free conditions with food and water ad libitum and a regular 12:12 light–dark cycle. Before the initiation of the experiments, animals were matched by body weight and fasting blood glucose (FBG) level (body weight reference range was from 17 to 23 g; FBG reference range was from 3.9 to 6.9 mM) then randomized into 3 groups (7–8 animals per group). For the blood glucose measurements, glucometer Contour Plus One (Ascensia Diabetes Care, Basel, Switzerland) was used. The animal experiment was performed in accordance with the EU Directive 2010/63/EU for animal experiments and was approved by the Institutional Ethics Board for Animal Care (National Medical Research Center for Cardiology, permit #385.06.2009).
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A commercially available glucometer (Contour Plus ONE; Ascensia Diabetes Care, Basel, Switzerland) was used to measure glucose levels in both blood and aqueous humor. Glucose concentration in the interstitial fluid was monitored continuously using a commercially available CGM system (FreeStyle Libre; Abbott Diabetes Care, CA, Alameda, USA). The sensor of the FreeStyle CGM system was implanted between the scapulae of the rabbit 1 hour before the start of the experiment for the sensor to fully calibrate. A smartphone was used to take measurements from the sensor using the Freestyle app via near-field communication.
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Glycated haemoglobin levels in capillary blood samples were measured immediately before the start of the tournament using the D-10 Haemoglobin A1c Program (Bio-Rad Laboratories, Hercules, CA, BioRad, Marnes-la-Coquette, France). Blood glucose levels were measured before and after each match by the medical team using glucose meters (Contour Plus One; Ascensia Diabetes Care). Capillary blood lactate levels were measured before and after each match using the Lactate Scout, EKF-Diagnostics (Manufacturer SensLab GmbH, Leipzig, Germany). The analysis of exercise blood lactate concentration was used to divide the players into two groups. The cut-off point was lactate concentration > 4 mmol/l which corresponds to OBLA (Onset Blood Lactate Accumulation). OBLA, which indicates the significant predominance of anaerobic mechanisms in ATP resynthesis, is known as the threshold load, and the measurement of OBLA is one of the methods for determining when the threshold load has been reached.
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