Aav dj camkiiα chr2 eyfp

The adeno-associated viruses AAV-DJ-CaMKIIα-ChR2-EYFP (genomic titer, 1 × 10¹² gc/mL) was acquired from the WZ Biosciences. Retro-AAV-hSyn-Cre (5 × 10¹² gc/mL), Retro-AAV-hSyn-mCherry (5 × 10^12;gc/mL), and AAV9-hSyn-ChrimsonR-tdTomato (5 × 10¹² gc/mL) were acquired from Brain VTA. All viral vectors were stored in aliquots at −80 °C until use.

The adeno-associated viruses AAV-DJ-EF1α-DIO-taCasp3 (genomic titer, 2 × 10¹³ gc/mL), AAV2-CAG-DIO-TVA-mCherry (7 × 10¹² gc/mL), AAV2-CAG-DIO-RG (8 × 10¹² gc/mL), Retro-AAV-hSyn-Cre (7 × 10¹³ gc/mL), AAV2-CaMKIIα-EYFP (8 × 10¹² gc/mL), AAV-DJ-CaMKIIα-ChR2-EYFP (8 × 10¹² gc/mL), AAV9-S5E2-Gq-tdTomato (1.8 × 10¹² gc/mL), AAV9-S5E2-tdTomato (5 × 10¹² gc/mL), and AAV9-EF1a-DIO-hChR2(H134R) (3 × 10¹² gc/mL) were acquired from the WZ Biosciences. AAV9-EF1α-DIO-GCaMP6s (5 × 10¹² gc/mL), AAV9-EF1a-DIO-hChR2(H134R)-EYFP (5 × 10¹² gc/mL), AAV9-EF1α-DIO-eNpHR3.0-EYFP (2 × 10¹² gc/mL), Retro-AAV-hSyn-EYFP (5 × 10¹² gc/mL) and Retro-AAV-hSyn-mCherry (5 × 10¹² gc/mL) were acquired from BrainVTA. AAV1-EF1α-DIO-Flp (1 × 10¹³ gc/mL), AAV9-EF1a-fDIO-EYFP (2 × 10¹² gc/mL), and AAV9-hSyn-SIO-eOPN3-mScarlet (1×10¹² gc/mL) were acquired from BrainCase. AAV9-EF1α-fDIO-GCaMP6s (5 × 10¹² gc/mL) and AAV9-hSyn-DIO-ChrimsonR-EYFP (3 × 10¹² gc/mL) was acquired from Taitool Bioscience. Glycoprotein-deleted (ΔG) and EnvA-pseudotyped rabies virus (RV-ΔG-EGFP+EnvA, 2 × 10⁸ IU/mL) was acquired from BrainVTA.