Gryphon lcp
The Gryphon LCP is a laboratory instrument designed for precision liquid chromatography. It provides accurate and consistent sample handling and analysis capabilities to support scientific research and testing applications.
7 protocols using gryphon lcp
Muscarinic Receptor Crystallization by LCP
Crystallization of Scribble PDZ1:TMIGD1 Complex
Crystallization of YeeE Protein
Structural Determination of Rh-PDE Crystals
Protein Crystallization Optimization Protocol
After one week, crystal growth was monitored under the microscope and plausible conditions were further tested at 18°C in 15-well plates (EasyXtal, USA) using the hanging-drop vapour-diffusion technique (1:1, 1:1.5 or 1:2 precipitant:protein ratios with 500 µl precipitant solution in the reservoir). Different PEG concentrations were tested in this screening. After one week, the best crystallization conditions were chosen. Crystals were found in two solutions, the first composed of 0.02 M KH2PO4, 0.1 M bis-Tris propane pH 6.5, 22% PEG 3350 and the second composed of 0.2 M ammonium acetate, 0.1 M bis-Tris pH 5.5, 20% PEG 3350. The crystals obtained in these conditions were cooled in liquid nitrogen in cryosolutions corresponding to the crystallization solutions supplemented with 20% glycerol.
Protein Crystal Growth via Sitting Drop
Protein Crystallization Optimization Protocol
After one week, crystal growth was monitored under the microscope and plausible conditions were further tested at 18°C in 15-well plates (EasyXtal, USA) using the hanging-drop vapour-diffusion technique (1:1, 1:1.5 or 1:2 precipitant:protein ratios with 500 µl precipitant solution in the reservoir). Different PEG concentrations were tested in this screening. After one week, the best crystallization conditions were chosen. Crystals were found in two solutions, the first composed of 0.02 M KH2PO4, 0.1 M bis-Tris propane pH 6.5, 22% PEG 3350 and the second composed of 0.2 M ammonium acetate, 0.1 M bis-Tris pH 5.5, 20% PEG 3350. The crystals obtained in these conditions were cooled in liquid nitrogen in cryosolutions corresponding to the crystallization solutions supplemented with 20% glycerol.
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