Accubind elisa

Demographic data collected from the medical records included age, weight, BMI (weight [kg] divided by height in meters squared [m ${}^2$ ]), obstetrics and gynecology history (duration of infertility and variability of menstrual cycles), and medications. Obesity was defined as BMI of $>$ 30 kg/m ${}^2$ and overweight as BMI of $>$ 25 kg/m ${}^2$ .
Physical and laboratory examinations (e.g., follicle-stimulating hormone [FSH], LH, thyroid-stimulating hormone [TSH], testosterone, prolactin, fasting blood sugar [FBS], and semen analyses of their husbands) were also performed. Impaired fasting blood sugar was defined as FBS of $>$ 100 mg/dL. The FSH and LH levels of the patients were measured on days 3 and 4 of the menstrual cycle. FSH, LH, testosterone, prolactin, and TSH levels were measured using AccuBindⓇ ELISA, Monobind Inc, USA. The normal ranges are as follows: TSH, 0.39-6.16 $\mu$ IU/mL; FSH, 3.0-12.0 mIU/mL in the follicular phase; LH, 5.0-10.5 mIU/mL in the follicular phase; testosterone, 0.2-0.95ng/mL; and prolactin, 1.2-19.5 ng/mL.
Vaginal ultrasonography (used to report endometrial thickness), hysterosalpingography, laparoscopy, and correlative endometrial biopsy reports were obtained from the patients' medical records.

Monobind AccuBind® ELISA Anti-SARS-CoV-2 kits were used as a qualitative determination of Anti-SARS-CoV-2 specific IgA, IgG and IgM antibodies at Drexel’s IMPACC site. These kits utilize a sequential sandwich ELISA method. This test utilizes recombinant nucleocapsid protein (rNCP) from SARS-CoV-2 coated on microwells to capture antibodies in human plasma. Patient plasma was diluted 1:100 and added directly to the ELISA plate. Following incubation and washing, IgA, IgG or IgM labeled antibodies were added. After a second incubation and wash, reagent substrate was added to produce a measurable color through the reaction with enzyme and hydrogen peroxide. After the addition of a stop substrate, absorbance was read in each well at 450 nm within 15 minutes of adding the stop solution.

Monobind AccuBind^® ELISA Anti-SARS-CoV-2 kits were used as a qualitative determination of Anti-SARS-CoV-2 specific IgA, IgG and IgM antibodies at Drexel’s IMPACC site. These kits utilize a sequential sandwich ELISA method. This test utilizes recombinant nucleocapsid protein (rNCP) from SARS-CoV-2 coated on microwells to capture antibodies in human plasma. Patient plasma was diluted 1:100 and added directly to the ELISA plate. Following incubation and washing, IgA, IgG or IgM labeled antibodies were added. After a second incubation and wash, reagent substrate is added to produce a measurable color through the reaction with enzyme and hydrogen peroxide. After the addition of a stop substrate, absorbance was read in each well at 450nm within 15 minutes of adding the stop solution.