Stat Fax 2100 Microplate Reader
The Stat Fax 2100 Microplate Reader is a compact and versatile instrument designed for absorbance-based assays. It can read microplates with up to 96 wells, utilizing a single-beam optical system with a tungsten-halogen light source. The reader is capable of performing absorbance measurements at a wide range of wavelengths, enabling a variety of applications in life science research and clinical diagnostics.
Lab products found in correlation
19 protocols using Stat Fax 2100 Microplate Reader
Evaluating 5-Azacytidine Cytotoxicity
Leptin Stimulates Cell Proliferation
Cell proliferation in each cell line was determined in triplicate for every concentration evaluated. After 24 h of stimulation, the cells were fixed with 1.1% glutaraldehyde (Merck, Germany) during 15 minutes and then PBS was added until the 96 h of stimulation with leptin was concluded for each group. At the end of stimulation, the fixed cells were stained with crystal-violet for 15 minutes, the plate was washed with water, and 10% acetic acid was placed in each well. Immediately after, the plate was read at an excitation wavelength of 490 nm and an emission wavelength of 630 nm in a microplate reader (Stat Fax 2100 Microplate Reader, Awareness Technology Inc.)
MTT Assay for Cell Viability
Multiplex Cytokine ELISA Assays
Evaluation of ATF3 Cytotoxicity via MTT Assay
Anthropometric and Hormonal Evaluation
BMI = body mass/ (height)2[kg/m2],
WHR = waist circumference (cm)/hip circumference (cm).
For measurement of the serum levels of follicle-stimulating hormone (FSH; Monobind kit, USA), luteinizing hormone (LH; Monobind kit, USA), testosterone (Monobind kit, USA), estrogen (Monobind kit, USA), insulin (Monobind kit, USA), and TNF-α (eBioscience, Austria), the enzyme-linked immunosorbent assay (ELISA) method was used according to the manufacturer’s recommendations. Color intensities at the final step were recorded using an ELISA reader (Stat Fax-2100 microplate reader, Awareness Technology, USA). The biochemical parameters including fasting blood glucose (Pars azmoon, Iran), triglyceride (Pars azmoon, Iran), total cholesterol (Pars azmoon, Iran), low-density lipoprotein (LDL; Pars azmoon, Iran), and high-density lipoprotein (HDL; Pars azmoon, Iran) were measured using a BT3000 autoanalyzer (Biotechnica Instruments, USA). Homeostasis model assessment (HOMA) as an insulin resistance index was computed using the following formula: HOMA = fasting glucose (mg/dl) × fasting insulin (mU/ml)/405.
Toxoplasma IgG ELISA Confirmation
Hormonal Profile Measurement Using ELISA
Quantifying Total Protein in Human Skim Milk
Dose-Dependent Tamoxifen Effects on Breast Cancer Cell Lines
At the end of the 48 hours of stimulation, the cells were fixed with 4% formaldehyde for 15 minutes. The fixed cells were washed with PBS and stained with crystal violet (0.02%) for 15 minutes, to remove the residual dye, 3 washes of 250 μl of water were carried out and finally 250 μl of 10% acetic acid was added to each well. The plate was read at an excitation wavelength of 490 nm and an emission length of 630 nm in a microplate reader (Stat Fax 2100 Microplate Reader, Awareness Technology Inc.).
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