Penicillin streptomycin pest

Name: Penicillin streptomycin pest
Brand: Merck Group
SKU: vCThCZIBPBHhf-iF4nto
Availability: InStock

Manufactured by Merck Group

7 citations

Sourced in United States

About the product

Penicillin-streptomycin (PEST) is a combination of two antibiotics, penicillin and streptomycin, commonly used in cell culture applications. It serves as a broad-spectrum antimicrobial agent to prevent bacterial contamination in cell and tissue cultures.

Automatically generated - may contain errors

Get Technical Specifications Find protocols

Market Availability & Pricing

Is this product still available?

Get pricing insights and sourcing options

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) A740003, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Caco 2, by American Type Culture Collection (1 mentions) Caco 2, by Lonza (1 mentions) L glutamine, by Lonza (1 mentions) Non essential amino acids (neaa), by Lonza (1 mentions) Plasmocin, by InvivoGen (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Mrc 5 human lung fibroblasts, by American Type Culture Collection (1 mentions) Insulin transferrin selenium, by Thermo Fisher Scientific (1 mentions) Dmem hg, by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Linoleic acid, by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Trypsin solution, by Merck Group (1 mentions) Atplite assay, by PerkinElmer (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions)

Check compatibility

7 protocols using «penicillin streptomycin pest»

Endocytic Pathway Modulation in MDCK Cells

2020

Cell Culture and Transfections MDCK II cells (hereafter referred to as MDCK) from American Type Cell Culture (ATCC CRL-2936, Rockville, MD) were grown in Dulbecco's Modified Eagle Medium (DMEM, low glucose, GlutaMAX™ Supplement, pyruvate) supplemented with 10% (v/v) fetal bovine serum (both from Thermo Fisher Scientific) and 1% (v/v) penicillinstreptomycin (PEST, Sigma Aldrich) at 37 C in 5% CO 2 . The cells were passaged at 80e90% confluency. To decipher the role of different endocytic pathways, major proteins known to be associated with these were knocked down using siRNA against either caveolin1 (HSS141467), GTPase regulator associated with focal adhesion kinase-1 (GRAF1, ARHGAP26HSS118162), clathrin heavy chain (clathrin HC, CLTCHSS102017, all from Thermo Fisher Scientific), Cdc42 (ON-TARGETplus siRNA human, J-005057-05, Dharmacon, Lafayette, CO, US) or scrambled control (Thermo Fisher Scientific). The cells were transfected twice with Lipofectamine 2000 and Opti-MEM (both from Thermo Fisher Scientific) according to the manufacturer's instructions over a period of 72 h before the uptake experiment. Protein levels of GRAF1 were determined previously, 31 while all others were analysed in this study by SDS-PAGE and immunoblotting using rabbit anti-caveolin1 antibody (Abcam, Cambridge, UK), mouse anti-clathrin heavy chain (clone 23, BD Transduction Laboratories, San Jose, CA, US) and rabbit anti-Cdc42 (Abcam). Mouse anti-b-actin (Sigma Aldrich) was used as loading control.

Wright L., Joyce P., Barnes T.J., Lundmark R., Bergström C.A.S., Hubert M, & Prestidge C.A. (2021). A Comparison of Chitosan, Mesoporous Silica and Poly(lactic-co-glycolic) Acid Nanocarriers for Optimising Intestinal Uptake of Oral Protein Therapeutics. Journal of pharmaceutical sciences, 110(1).

+ Open protocol

+ Expand

Corresponding organizations : University of South Australia, Umeå University, Uppsala University

Evaluating Cytotoxicity of Coaxial NFs

Cited 1 time 2020

To investigate the potential biological application of the coaxial GDD NFs, in vitro cytotoxicity study was done for GDD, the plain and medicated coaxial NFs using methyl thiazolyl tetrazolium (MTT) assay. A Human colorectal adenocarcinoma (Caco-2) was obtained from the American Type Culture Collection (Manassas, VA, USA). Caco-2 cells were cultured in complete cell culture medium (DMEM+) composed of: Dulbecco Modified Eagle’s Minimal Essential medium (DMEM; 4.5 g/L glucose) supplemented with L-glutamine (Lonza, Belgium), non-essential amino acids (NEAA; 1% v/v; BioWhittaker, Lonza), penicillin-streptomycin (PEST; 1% v/v; Sigma, MO, USA), and heat-inactivated fetal bovine serum (FBS; 10% v/v; Gibco, MA, USA). Culture medium was refreshed every two day (at 80–85% confluence) using 0.25% trypsin and 0.02% ethylenediaminetetraacetic acid (EDTA).

Darwesh A.Y., El-Dahhan M.S, & Meshali M.M. (2020). New Oral Coaxial Nanofibers for Gadodiamide-Prospective Intestinal Magnetic Resonance Imaging and Theranostic. International Journal of Nanomedicine, 15, 8933-8943.

+ Open protocol

+ Expand

Corresponding organizations : Mansoura University

Isolation and Culture of Human Gingival Fibroblasts

2019

Mouse C2C12 mouse satellite cells were provided by Prof. Anna Starzinski-Powitz (Goethe-Universität, Frankfurt am Main, Germany) and Phoenix 293 cells were provided by Prof. James Lorens, University of Bergen. Primary human gingival fibroblasts (hGF) were isolated from healthy gingival tissues as described earlier^{59 (link)}. MRC5 human lung fibroblasts (American Type Culture Collection) were obtained from Robert Lafyatis laboratory (University of Pittsburgh Medical Center, Pittsburgh, PA, USA). Cells were cultured at 37 °C in Dulbecco’s modified Eagle’s medium (DMEM; Gibco®, Invitrogen) with 10% fetal bovine serum (FBS; Gibco®, Invitrogen), 1% penicillin-streptomycin (PEST; Sigma-Aldrich) and 5 µg/ml plasmocin (InvivoGen). Human gingival fibroblasts were grown from biopsies obtained during oral surgery after obtaining informed consent and in accordance with guidelines and regulations at the Department of Prosthetic Dentistry, Karolinska Institute, Stockholm in the 1990s following approval of experimental protocols by local ethics committee at faculty of Odontology, Karolinska institute and were kindly provided by Prof. Kamal Mustafa (University of Bergen)^{60 (link)}.

Erusappan P., Alam J., Lu N., Zeltz C, & Gullberg D. (2019). Integrin α11 cytoplasmic tail is required for FAK activation to initiate 3D cell invasion and ERK-mediated cell proliferation. Scientific Reports, 9, 15283.

+ Open protocol

+ Expand

Corresponding organizations : Oslo University Hospital, University of Oslo, Institutt for Eksperimentell Medisinsk Forskning, University of Bergen, Princess Margaret Cancer Centre, University Health Network

Culturing Rat Cortical and Hippocampal Neurons

2018

All animal experiments were approved by the animal experiments ethics committee at the Central Research Institute of Electric Power Industry (CRIEPI), and were performed according to the guidelines for the care and use of laboratory animals. In addition, we observed the principles of animal welfare and conducted experiments with the minimum possible number of animals. Isolation and culture of cortical and hippocampal neurons and astrocytes were performed with the following methods, based on a previous report (Saito et al., 2017 (link)). Briefly, cortical and hippocampal tissues harvested from 18- to 19-day-old Wistar rat embryos (Charles River Laboratories, Japan) were enzymatically dissociated to single cells using 0.5%, 15–20 min Trypsin solution (Sigma-Aldrich, St. Louis, MO) treatment. Dulbecco's Modified Eagle Medium (DMEM, high glucose; Thermo Fisher Scientific, Waltham, MA) containing 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 5% horse serum (HS, Thermo Fisher Scientific), and 1% Penicillin-Streptomycin (Pe-St, Sigma-Aldrich) was used for the cell culture medium. To detect the neuronal network activity, we used a multi-electrode array (MEA) dish, which has 50 μm-diameter circle electrodes separated by 150 mm intervals and were arranged in 8 × 4 separated blocks (MED-P5003A; Alpha MED Scientific, Osaka, Japan) (Figure 1A). The center distance between the 8 × 4 separated blocks was 12 mm; this distance is necessary to enhance the effect of the magnetically induced current generated concentrically. The isolated neuronal and glial cells were seeded on the MEA dish with a density of 30 × 10⁴ cells and 2 mm-diameter in each separated block. During the culture, half of the medium was exchanged with fresh medium twice a week to maintain culture conditions.

Saito A., Takahashi M., Makino K., Suzuki Y., Jimbo Y, & Nakasono S. (2018). Response of Cultured Neuronal Network Activity After High-Intensity Power Frequency Magnetic Field Exposure. Frontiers in Physiology, 9, 189.

+ Open protocol

+ Expand

Corresponding organizations : Central Research Institute of Electric Power Industry, Tokyo Metropolitan University, The University of Tokyo

Chondrocyte Expansion and Differentiation

2018

Chondrocytes was expanded in monolayer as earlier described [42] (link). Subsequently passage 3 cells were seeded at 20, 000 cells/cm² in Dulbecco’s modified Eagles medium-high glucose (DMEM-HG) (Thermo Fisher Scientific; Waltham, MA, USA) supplemented with 14 μg/ml ascorbic acid (Sigma-Aldrich, St. Louis, MO, USA), 10⁻⁷ M dexamethasone (Sigma-Aldrich), 1 mg/mL human serum albumin (Equitech Bio, Kerville, TX, USA), 1 × insulin–transferrin–selenium (Gibco, Life Technologies, Carlsbad, CA, USA), 5 μg/mL linoleic acid (Sigma-Aldrich), 1 × penicillin-streptomycin (PEST) (Sigma-Aldrich), and 10 ng/mL human transforming growth factor (TGF) β-1 (R&D Systems, Abingdon, UK) on glass coverslips (Nr 1, diameter 20 mm, BergmanLabora, Stockholm, Sweden) in 12 well culture plates for Ca²⁺ and immunohistochemistry analysis, or in 6 well culture plates for Western blot analysis.

Skiöldebrand E., Thorfve A., Björklund U., Johansson P., Wickelgren R., Lindahl A, & Hansson E. (2018). Biochemical alterations in inflammatory reactive chondrocytes: evidence for intercellular network communication. Heliyon, 4(1), e00525.

+ Open protocol

+ Expand

Corresponding organizations : Sahlgrenska University Hospital, University of Gothenburg

Top 2 most cited protocols using «penicillin streptomycin pest»

Cytotoxicity Assessment of Zeolite Nanoparticles

Fifteen milligrams of lyophilized native
zeolite K-LTL, Gd-LTL, and PEG-Gd-LTL NPs were suspended in 1 mL of
filter-sterile water containing 0.05% bovine serum albumin (BSA).
These stock suspensions were sonicated for 10 min at 20 °C in
a Branson 5510 water bath sonicator (Emerson, USA) at 100% output
(4W specific ultrasound energy, 240 J/m³). The suspensions
were transferred into glass vials and the total volume was adjusted
to 3 mL with 0.05% BSA (the final concentration of the stock suspensions
was 5000 μg mL^–1). Mouse macrophage cells
RAW 264.7 (ATCC TIB-71) were obtained from the American Type Culture
Collection, (ATCC, Manassas, VA). Cells were used at passages 8 and
10. RAW 264.7 cells were cultured in Dulbecco’s Modified Eagle’s
Medium (DMEM; 4.5 g L^–1 glucose, w/o l-glutamine,
w/o phenol red, Lonza, Verviers, Belgium) supplemented with glutamax
(Gibco). Also, penicillin-streptomycin (PEST; 1% v/v; Sigma, St. Louis,
MO), and heat-inactivated fetal bovine serum (FBS; 10% v/v; Gibco)
were added to the medium. Cells were incubated at 37 °C in a
humidified environment (95% relative humidity) and 5% carbon dioxide
with a Thermo Scientific HERAcell 240. Cells were passed at 80% confluency
by subcultivation at a 1:6 ratio twice a week until use. All media
and solutions were prewarmed to 37 °C before use.
The viability
was assessed using the ATPlite assay (PerkinElmer). 100 μL suspensions
with a concentration of 2 × 10⁴ cells/mL were seeded
in 96 wells white plates with clear bottom (Costar) (6250 cells/well).
After 48 h, these cells reached a confluency of 30%. To assess the
possible cytotoxic effect of the NPs on RAW264.7 cells, a dilution
range of 10, 25, 50, 100, 250, and 500 μg/mL nanoparticles (corresponding
to 2.87, 7.17, 14.3, 28.7, 71.7, and 143.4 μM Gd) was added
to the cells for 24 h. Next, 50 μL of mammalian cell lysis solutions
were added to 100 μL of cell suspension, and incubated for 5
min in an orbital shaker at 700 rpm. Subsequently, 50 μL of
substrate solution were added, followed by shaking for another 5 min
at 700 rpm, and a final incubation in the dark for 10 min. Chemoluminescence
was measured at 590 nm. A control of NPs without cells, showed no
interference of the NPs with the ATPlite assay.

Zhang W., Martinelli J., Peters J.A., van Hengst J.M., Bouwmeester H., Kramer E., Bonnet C.S., Szeremeta F., Tóth É, & Djanashvili K. (2017). Surface PEG Grafting Density Determines Magnetic Relaxation Properties of Gd-Loaded Porous Nanoparticles for MR Imaging Applications. ACS Applied Materials & Interfaces, 9(28), 23458-23465.

+ Open protocol

+ Expand

Corresponding organizations : Delft University of Technology, Wageningen University & Research, Centre National de la Recherche Scientifique, Centre de Biophysique Moléculaire, Le Studium

Quantifying Inflammasome Activation in Toxoplasma Infection

T. gondii type-I RH strain expressing yellow fluorescence protein (YFP) 39 (link) and type-II Prugniaud (PRU) expressing tandem dimers of tomato red (tdTomato) fluorescent protein T. gondii strain was used in our experiments 40 (link). The tachyzoites were harvested by serial 4-5 day passage in human foreskin fibroblast (HFF) cultured in DMEM (Sigma-Aldrich) with 10% FBS (Sigma-Aldrich) and 1% penicillin/streptomycin (PEST) (Sigma-Aldrich). The mouse colonic epithelial cell line, CMT-93, was maintained in DMEM (Sigma-Aldrich) media with 10% FBS (Sigma-Aldrich), 2mM L-glutamine (Sigma-Aldrich) and 1% penicillin/streptomycin (PEST) (Sigma-Aldrich). CMT-93 cells were infected with PRU at a ratio of 1:5 for 24 h and treated with the selective P2X7R antagonist, A-740003 (500μM; Sigma-Aldrich) to block P2X7R at the time of infection. 1x10⁶ cells per group) cells were infected by T. gondii PRU strain in a ratio of 1:1. THP-1 monocyte cells were primed using 0.1 ug/ml of LPS for 24h followed by infection with T. gondii for 24h in a ratio of 1:4 .
Immortalized murine bone marrow-derived macrophages (iBMDMs) were obtained from Prof Claire Bryant (Department of Veterinary Medicine, University of Cambridge). iBMDMs and CMT-93 epithelial cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), 10 % fetal bovine serum (FBS, Life Technologies), 100 U/ml penicillin and 100 µg/ml streptomycin (PenStrep). Cells were seeded overnight at 5 x 10⁵ cells/ml (iBMDMs) in 24 well plates. Cells were primed with LPS (1μg/ml, 2h). Following priming inflammasomes were stimulated by adding ATP (5 mM) for 1 h. Supernatants were removed and analyzed for IL-1β content by ELISA (DuoSet, R&D systems) according to manufacturer’s instructions.

Huang S.W., Walker C., Pennock J., Else K., Muller W., Daniels M.J., Pellegrini C., Brough D., Lopez-Castejon G, & Cruickshank S.M. (2016). P2X7 receptor-dependent tuning of gut epithelial responses to infection. Immunology and Cell Biology, 95(2), 178-188.

+ Open protocol

+ Expand

Corresponding organizations : University of Manchester, University of Pisa

Spelling variants (same manufacturer)

Streptomycin - 17516 citations Penicillin - 16503 citations Penicillin/streptomycin - 14500 citations

Similar products (other manufacturers)

The spelling variants listed above correspond to different ways the product may be referred to in scientific literature.
These variants have been automatically detected by our extraction engine, which groups similar formulations based on semantic similarity.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!