Mouse C2C12 mouse satellite cells were provided by Prof. Anna Starzinski-Powitz (Goethe-Universität, Frankfurt am Main, Germany) and Phoenix 293 cells were provided by Prof. James Lorens, University of Bergen. Primary human gingival fibroblasts (hGF) were isolated from healthy gingival tissues as described earlier59 (link). MRC5 human lung fibroblasts (American Type Culture Collection) were obtained from Robert Lafyatis laboratory (University of Pittsburgh Medical Center, Pittsburgh, PA, USA). Cells were cultured at 37 °C in Dulbecco’s modified Eagle’s medium (DMEM; Gibco®, Invitrogen) with 10% fetal bovine serum (FBS; Gibco®, Invitrogen), 1% penicillin-streptomycin (PEST; Sigma-Aldrich) and 5 µg/ml plasmocin (InvivoGen). Human gingival fibroblasts were grown from biopsies obtained during oral surgery after obtaining informed consent and in accordance with guidelines and regulations at the Department of Prosthetic Dentistry, Karolinska Institute, Stockholm in the 1990s following approval of experimental protocols by local ethics committee at faculty of Odontology, Karolinska institute and were kindly provided by Prof. Kamal Mustafa (University of Bergen)60 (link).
Penicillin streptomycin pest
Manufactured by Merck Group
Sourced in United States
Penicillin-streptomycin (PEST) is a combination of two antibiotics, penicillin and streptomycin, commonly used in cell culture applications. It serves as a broad-spectrum antimicrobial agent to prevent bacterial contamination in cell and tissue cultures.
Automatically generated - may contain errors
Lab products found in correlation
Plasmocin, by InvivoGen (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Mrc 5 human lung fibroblasts, by American Type Culture Collection (1 mentions)
Non essential amino acids (neaa), by Lonza (1 mentions)
Caco 2, by American Type Culture Collection (1 mentions)
L glutamine, by Lonza (1 mentions)
Caco 2, by Lonza (1 mentions)
Insulin transferrin selenium, by Thermo Fisher Scientific (1 mentions)
Dmem hg, by Thermo Fisher Scientific (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Human serum albumin (hsa), by Equitech-Bio (1 mentions)
3 protocols using penicillin streptomycin pest
1
Isolation and Culture of Human Gingival Fibroblasts
2
Evaluating Cytotoxicity of Coaxial NFs
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To investigate the potential biological application of the coaxial GDD NFs, in vitro cytotoxicity study was done for GDD, the plain and medicated coaxial NFs using methyl thiazolyl tetrazolium (MTT) assay. A Human colorectal adenocarcinoma (Caco-2) was obtained from the American Type Culture Collection (Manassas, VA, USA). Caco-2 cells were cultured in complete cell culture medium (DMEM+) composed of: Dulbecco Modified Eagle’s Minimal Essential medium (DMEM; 4.5 g/L glucose) supplemented with L-glutamine (Lonza, Belgium), non-essential amino acids (NEAA; 1% v/v; BioWhittaker, Lonza), penicillin-streptomycin (PEST; 1% v/v; Sigma, MO, USA), and heat-inactivated fetal bovine serum (FBS; 10% v/v; Gibco, MA, USA). Culture medium was refreshed every two day (at 80–85% confluence) using 0.25% trypsin and 0.02% ethylenediaminetetraacetic acid (EDTA).
3
Chondrocyte Expansion and Differentiation
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Chondrocytes was expanded in monolayer as earlier described [42] (link). Subsequently passage 3 cells were seeded at 20, 000 cells/cm2 in Dulbecco’s modified Eagles medium-high glucose (DMEM-HG) (Thermo Fisher Scientific; Waltham, MA, USA) supplemented with 14 μg/ml ascorbic acid (Sigma-Aldrich, St. Louis, MO, USA), 10−7 M dexamethasone (Sigma-Aldrich), 1 mg/mL human serum albumin (Equitech Bio, Kerville, TX, USA), 1 × insulin–transferrin–selenium (Gibco, Life Technologies, Carlsbad, CA, USA), 5 μg/mL linoleic acid (Sigma-Aldrich), 1 × penicillin-streptomycin (PEST) (Sigma-Aldrich), and 10 ng/mL human transforming growth factor (TGF) β-1 (R&D Systems, Abingdon, UK) on glass coverslips (Nr 1, diameter 20 mm, BergmanLabora, Stockholm, Sweden) in 12 well culture plates for Ca2+ and immunohistochemistry analysis, or in 6 well culture plates for Western blot analysis.
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