D mannitol

Manufactured by Merck Group

Sourced in United States, United Kingdom, Germany, Canada, Spain, Italy, Japan, France

D-mannitol is a type of sugar alcohol commonly used in the production of pharmaceutical and laboratory equipment. It serves as a bulking agent, sweetener, and excipient in various formulations. D-mannitol is a white, crystalline powder with a sweet taste and is soluble in water. It is widely utilized in the pharmaceutical and biotechnology industries as a component in drug tablets, capsules, and other medicinal products.

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Lab products found in correlation

D glucose, by Merck Group (51 mentions) Sucrose, by Merck Group (22 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (16 mentions) Bovine serum albumin (bsa), by Merck Group (14 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (13 mentions) Dimethyl sulfoxide (dmso), by Merck Group (12 mentions) D fructose, by Merck Group (10 mentions) Sodium chloride (nacl), by Merck Group (10 mentions) D sorbitol, by Merck Group (9 mentions) D trehalose dihydrate, by Merck Group (9 mentions) Potassium chloride (kcl), by Merck Group (9 mentions) Sodium succinate, by Merck Group (7 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (7 mentions) D xylose, by Merck Group (7 mentions) Polyvinyl alcohol (pva), by Merck Group (7 mentions) Tween 80, by Merck Group (6 mentions) Magnesium stearate, by Merck Group (6 mentions) D galactose, by Merck Group (6 mentions) Thiobarbituric acid, by Merck Group (6 mentions) L leucine, by Merck Group (6 mentions)

246 protocols using d mannitol

Apoptosis Signaling Pathway Assay

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Quinoyl-valyl-O-methylaspartyl-(2,6-difluoro-phenoxy)-methyl ketone or Q-VD-OPh, everolimus, propidium iodide (PI), cycloheximide (CHX) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France), LY294002 from Biomol (Hamburg, Germany), bortezomib from Selleckchem (Houston, TX), MG-132 from Calbiochem (Gibbstown, NJ), DZNep from Cayman Chemical, (Ann Arbor, MI). Drugs were dissolved in ethanol (EtOH) or DMSO to obtain stock solutions (10–50 mM) and were diluted in serum-free culture medium before use. For control experiments using drugs, ethanol (EtOH) or dimethylsulfoxide (DMSO) were added as vehicles at the same concentration. For in vivo experiments, DZNep was dissolved in 10% D-mannitol (Sigma-Aldrich), then diluted at the appropriate concentration in PBS to reach 0.1% D-mannitol for i.p mice injections.
The following antibodies (Abs) were used in the study: anti-β-actin (sc-47778), anti-caspase 3 (sc-7148), and anti-caspase 8 (sc-7890) from Santa Cruz Biotechnologies (Santa Cruz, CA); anti-caspase 9 (#9508), anti-poly (ADP-ribose) polymerase or PARP (#9542) and anti-EZH2 (#3147) from Cell Signaling Technology (Danvers, MA); anti-B-cell lymphoma 2 or BCL2 (110887) from Dako (Courtaboeuf, France), anti-glyceraldehyde-3-phosphodeshydrogenase (GAPDH, #4300) from Applied Biosystems/Ambion (Austin, TX) and anti-H3K27me3 (mAb6002) from Abcam (Paris, France).

Gaudichon J., Milano F., Cahu J., DaCosta L., Martens A.C., Renoir J.M, & Sola B. (2014). Deazaneplanocin A Is a Promising Drug to Kill Multiple Myeloma Cells in Their Niche. PLoS ONE, 9(9), e107009.

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Mannitol Solute Concentration Effects

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D-Mannitol was purchased from Sigma Aldrich (type number M4125), and different solute concentrations of the solution (D-Mannitol and distilled water), 0.12 M (0.3 MPa), 0.24 M (0.6 MPa), 0.36 M (0.9 MPa), 0.48 M (1.2 MPa) and 0.61 M (1.5 MPa), were prepared. The numbers in parentheses are the associated solute potentials ( s ψ ) which are calculated using s icRT ψ = -
, where i = 1 for mannitol, c is the solute concentration (unit is mol/L), R is the pressure constant (8.314 kPa•L/mol•K) and T is the absolute temperature.

Li W., Keynia S., Belteton S.A., Hatam F.A., Szymanski D.B., & Turner J.A. (2021). Protocol for mapping the spatial variability in cell wall mechanical bending behavior in living leaf pavement cells.

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Mannitol Osmotic Potential Preparation

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D-Mannitol was purchased from Sigma Aldrich (type number M4125), and different solute concentrations of the solution (D-Mannitol and distilled water), 0.12 M (0.3 MPa), 0.24 M (0.6 MPa), 0.36 M (0.9 MPa), 0.48 M (1.2 MPa), and 0.61 M (1.5 MPa), were prepared. The associated solute potentials, ψ , shown in parentheses, were calculated using ψ = -icRT, where i = 1 for mannitol, c is the solute concentration (unit is mol/L), R is the pressure constant (8.314 kPa•L/mol•K), and T is the absolute temperature in degrees Kelvin.

Li W., Keynia S., Belteton S.A., Afshar-Hatam F., Szymanski D.B, & Turner J.A. (2022). Protocol for mapping the variability in cell wall mechanical bending behavior in living leaf pavement cells. Plant physiology, 188(3).

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Rat Trophoblast Stem Cells under Hyperglycemia

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Blastocyst-derived rat TS cells15 (link) were cultured in basal culture medium (RPMI 1640 (Cellgro), 20% fetal bovine serum (Sigma-Aldrich), 100 µM 2-mercaptoethanol (M7522; Sigma-Aldrich), 1 mM sodium pyruvate (ThermoFisher, 11 360–070), 50 µM penicillin (15140122; ThermoFisher), and 50 U/mL streptomycin (15140122; ThermoFisher)) supplemented with 70% rat embryonic fibroblast-conditioned medium, fibroblast growth factor 4 (25 ng/mL; Sigma-Aldrich), and heparin (1 µg/mL; Sigma-Aldrich). To model hyperglycemia, 25 mM glucose (Sigma-Aldrich) was added to the culture medium. D-mannitol (Sigma-Aldrich) was added to the culture medium to control for osmolality. Rat TS cells were exposed to ambient or low oxygen (0.5% or 1.5% O₂) tensions and 5% CO₂ at 37°C in an NAPCO 8000 incubator (ThermoFisher) for 24 hours and then harvested for immunocytochemical or biochemical analyses.

Nteeba J., Varberg K.M., Scott R.L., Simon M.E., Iqbal K, & Soares M.J. (2020). Poorly controlled diabetes mellitus alters placental structure, efficiency, and plasticity. BMJ Open Diabetes Research & Care, 8(1), e001243.

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Metabolite Profiling of Biological Samples

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All solvents were high purity high-performance liquid chromatography (HPLC) grade purchased from J.T. Baker (Philipsburg, NJ, USA). Distilled water was filtered using a Milli-Q Reagent Water System (Millipore, Billerica, MA, USA). Methyloctanoate, succinic acid-d₄, methoxyamine hydrochloride, pyridine, sodium sulfate, and boron trifluoride-methanol were purchased from Sigma-Aldrich (St. Louis, MO, USA). The certified reference material (CRM), a 37 fatty acid methyl ester mixture, and N-methyl-N-(trimethylsilyl)trifluoroacetamide with trimethylchlorosilane as a silylation reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). The standards of benzeneethanol, benzaldehyde, 1H-pyrrole-2-carboxaldehyde, γ-butyrolactone, γ-caprolactone, δ-hexalactone, γ-octalactone, ethylene glycol, l-(−)-arabitol, d-mannitol, scyllo-inositol, d-glyceric acid sodium salt, fumaric acid, d-psicofuranose, d-gluconic acid solution, and succinic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-Octen-2-one, pantolactone, malic acid, and azelaic acid were obtained from Supelco (Bellefonte, PA, USA).

Kim H., Jung Y., Jeon S.H., Hwang G.S, & Ahn Y.G. (2019). Rapid Characterization and Discovery of Chemical Markers for Discrimination of Xanthii Fructus by Gas Chromatography Coupled to Mass Spectrometry. Molecules, 24(22), 4079.

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Formulation and Evaluation of Ibuprofen ODT

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Nurofen Meltlets (A marketed ibuprofen 200 mg ODT) and standard immediate release paracetamol tablets were obtained from Reckitt Benckiser (Slough, UK) and Wockhardt (Wrexham, UK) respectively.
Materials for placebo ODT preparation included D-mannitol (Sigma-Aldrich, UK), Microcrystalline Cellulose (MCC) (Avicel PH102, FMC Biopolymer, USA), Partially Pregelatinised Maize Starch (Starch 1500®, Colorcon Inc., USA), crospovidone (Kollidon CL, BASF, Germany), Pearlitol Flash (Roquette, France), sodium stearyl fumarate (Alubra, FMC Biopolymer, USA) and magnesium stearate (Fischer Scientific, UK). Hydroxypropyl methylcellulose (HPMC) (METHOCEL™ K100M, provided by Colorcon Inc., UK) was used to manufacture hydrophilic matrix tablets.

Koner J.S., Rajabi-Siahboomi A.R., Missaghi S., Kirby D., Perrie Y., Ahmed J, & Mohammed A.R. (2019). Conceptualisation, Development, Fabrication and In Vivo Validation of a Novel Disintegration Tester for Orally Disintegrating Tablets. Scientific Reports, 9, 12467.

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Synthesis of Iron Oxide Nanoparticles

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Iron(II) chloride tetrahydrate (FeCl2·4H2O), iron(III) chloride hexahydrate (FeCl3·6H2O), and d-mannitol were purchased from Sigma–Aldrich (St. Louis, MO). Ammonium hydroxide (NH4OH) was obtained from EMD chemicals (Gibbstown, NJ). Chloroform, anhydrous methanol, and high performance liquid chromatography-grade methanol were purchased from Fisher Scientific (Pittsburgh, PA). Ultrahigh-purity nitrogen gas was purchased from Scott-Gross (Lexington, KY).

Stocke N.A., Sethi P., Jyoti A., Chan R., Arnold S.M., Hilt J.Z, & Upreti M. (2016). Toxicity evaluation of magnetic hyperthermia induced by remote actuation of magnetic nanoparticles in 3D micrometastasic tumor tissue analogs for triple negative breast cancer. Biomaterials, 120, 115-125.

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Antioxidant Effects on Biofilm Electrocidal

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Biofilms were grown on discs in the presence and absence of antioxidants. Standard phosphate flow buffer or buffer supplemented with antioxidant was used. Catalase from bovine liver (Sigma, St. Louis, MO) was reconstituted in potassium phosphate buffer. The free radical scavengers D-mannitol (Sigma) and Tempol (Sigma) were reconstituted in water. Catalase was placed into the flow buffer at a final concentration of either 200 or 500 U/ml. D-mannitol was placed into the flow buffer at a final concentration of 20 or 50 mM and Tempol was placed into the flow buffer at a concentration of 1 mM. Additionally, biofilms were grown on a set of discs in TSB supplemented with 500 U/ml of catalase, 20 mM of mannitol, or 5 or 10 mM of Tempol. The discs were exposed to 20 or 200 μA DC or no current for 24 hours using a 3% phosphate buffer with or without antioxidant. The LRF from samples supplemented with antioxidants was subtracted from the LRF from samples with no antioxidant supplementation; a positive number indicates protection against the electricidal effect with a >0.5 change in the LRF considered protective.

Brinkman C.L., Schmidt-Malan S.M., Karau M.J., Greenwood-Quaintance K., Hassett D.J., Mandrekar J.N, & Patel R. (2016). Exposure of Bacterial Biofilms to Electrical Current Leads to Cell Death Mediated in Part by Reactive Oxygen Species. PLoS ONE, 11(12), e0168595.

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SRBC Conjugation and Immunization

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HEL proteins were desalted into conjugation buffer (0.35 M D-mannitol [Sigma] and 0.01 M sodium chloride [Sigma]) using PD-10 columns (Amersham) as described previously (38 (link), 70 (link)). For conjugation, SRBCs were washed three times in phosphate-buffered saline (PBS) and once in conjugation buffer as described previously (38 (link)). SRBCs were then resuspended in a final volume of 1 mL conjugation buffer containing 10 µg/mL. The solution was mixed on a platform rocker on ice for 10 min. One hundred microliters of 100 mg/mL N-(3-dimethylaminopropyl)-N-ethylcarbodimide hydrochloride (Sigma) was then added and the solution was mixed for a further 30 min on ice. SRBCs were then washed four times in PBS. Confirmation of successful conjugation was performed by flow-cytometric analysis of SRBCs using Alexa Fluor 647-conjugated Hy9 antibody. A total of 2 × 10⁸ conjugated or unconjugated SRBCs were i.v. injected into each chimeric mouse.

Burnett D.L., Schofield P., Langley D.B., Jackson J., Bourne K., Wilson E., Porebski B.T., Buckle A.M., Brink R., Goodnow C.C, & Christ D. (2020). Conformational diversity facilitates antibody mutation trajectories and discrimination between foreign and self-antigens. Proceedings of the National Academy of Sciences of the United States of America, 117(36), 22341-22350.

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Evaluating IGF-1R Signaling Pathways

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Total IGF-1R, IRS-1, p85, ERK1/2 and Akt and phosphorylated (Tyr1135/1136) IGF-1R, (Tyr458) p85, (Thr202/Tyr204) ERK1/2, (Ser473) Akt, and GAPDH antibody (rabbit) primary antibodies were obtained from Cell Signaling (Danvers, MA). Secondary goat anti-rabbit IgG antibodies (IRDye 800CW) were purchased from LI-COR Biotechnology (Lincoln, NE). AG 1024, a specific IGF-1R phosphorylation inhibitor, was purchased from Selleck Chemicals (Houston, TX). IGF-1R siRNA was obtained from Cell Signaling (Danvers, MA). D-mannitol and glucose were purchased from Sigma (St. Louis, MO). KZ-41 (Fig 1) was synthesized in Dr. Duane Miller’s laboratory and verified to be >96% pure by nuclear magnetic resonance spectroscopy [17 (link)].

He H., Weir R.L., Toutounchian J.J., Pagadala J., Steinle J.J., Baudry J., Miller D.D, & Yates C.R. (2017). The quinic acid derivative KZ-41 prevents glucose-induced caspase-3 activation in retinal endothelial cells through an IGF-1 receptor dependent mechanism. PLoS ONE, 12(8), e0180808.

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