Sw480

Manufactured by American Type Culture Collection

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About the product

The SW480 is a laboratory equipment product from the American Type Culture Collection (ATCC). It is a colorectal adenocarcinoma cell line that can be used for various cell-based research applications.

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The SW480 cell line is officially offered by the American Type Culture Collection (ATCC) and available through authorized distributors. The price for a single unit is approximately $555.

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SW480 cells (ATCC® CCL-228™, (3 citations) SW480 colon cancer cells (3 citations) HCT116 and SW480 (2 citations) SW480 colon adenocarcinoma (2 citations) SW480 primary tumour-derived cells (CCL-228) (1 citations) SW480 colorectal cancer (1 citations) SW480 primary colon cancer cells (CCL-228) (1 citations) SW480 (KRAS G12V) (1 citations) HT-29 and SW480 (1 citations) SW480 ATCC (1 citations)

The spelling variants listed below correspond to different ways the product may be referred to in scientific literature.
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2 883 protocols using sw480

Exploring Cell Line Responses to Autophagy and Cell Death Modulators

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Human colorectal cancer (CRC) cell lines (HCT116, HT-29, SW480, LoVo, and DLD-1), a normal colon fibroblast cell line (CCD-18Co), and human non-small cell lung carcinoma (NSCLC) cell lines (HCC827, H820, H1975, H460, and A549) were obtained from the American Type Culture Collection (Manassas, VA, USA). The CRC cell lines SNU-70 and SNU-796 were purchased from the Korea Cell Line Bank (Seoul, South Korea). CCD18Co cells were cultured in DMEM (WELGENE, Daegu, South Korea), while CRC and NSCLC cells were cultured in RPMI 1640 (WELGENE) supplemented with 10% fetal bovine serum (WELGENE) and 1% penicillin/streptomycin (WELGENE) at 37 °C in a humidified atmosphere containing 5% CO₂. Bafilomycin A1 (BA1) (B1793, Sigma-Aldrich, St. Louis, MI, USA), Z-VAD-FMK (S7023, Selleckchem, USA), ferrostatin-1 (S7243, Selleckchem), necrostatin-1 (S8037, Selleckchem), etoposide (S1225, Selleckchem), cycloheximide (CHX) (S7418, Selleckchem), MG132 (474787, Sigma-Aldrich), leupeptin hemisulfate (S7380, Selleckchem), tunicamycin (T7765, Sigma-Aldrich) were dissolved in dimethyl sulfoxide (DMSO) (D2650, Sigma-Aldrich). Iron (III)-citrate (F3388; Sigma-Aldrich) was dissolved in distilled water. Cells were seeded in an appropriate culture dish. After overnight incubation, cells were treated with DMSO, or BA1 (1.5–2 nM) with or without Iron (III)-citrate (10 µM), or other indicated chemicals.

Min D.H., Kim D., Hong S.T., Kim J., Kim M.J., Kwon S., kim A, & Lee J.Y. (2025). Bafilomycin A1 induces colon cancer cell death through impairment of the endolysosome system dependent on iron. Scientific Reports, 15, 5148.

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Modulation of NQO1 Regulates Colorectal Cancer Metastasis

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Colorectal cancer cell lines, namely SW480, SW620, HCT-116, and DLD-1, alongside the normal colonic epithelial cell line HCoEpic, were acquired from the American Type Culture Collection (ATCC) and subsequently propagated. Cultivation of these cells was conducted using RPMI 1640 Medium (supplied by Gibco Laboratories, Grand Island, NY), enriched with 10% fetal bovine serum (FBS) (also sourced from Gibco), 100 U/mL of penicillin, and 100 µg/mL of streptomycin. This was performed at a controlled temperature of 37 °C within an atmosphere containing 5% CO2. For the generation of stable cell lines, lentiviral vectors were utilized to modulate NQO1 expression, either enhancing or silencing its activity (provided by Genechem, Shanghai, China). Post-transfection, cells were subjected to selection via 2 µg/ml puromycin over two weeks. MicroRNA agents, including the miR-484-5p mimic, a nonspecific miRNA control, a miR-485-5p inhibitor, and a nonspecific miRNA inhibitor control, were obtained from RIBOBIO (China). In adherence to the supplier’s protocol, transfections of miRNA were executed employing Lipofectamine 3000 reagent within Opti-MEM reduced serum medium (courtesy of Invitrogen, USA).
Fig. 3

NQO1 promoted the metastasis of CRC cells via EMT process in vitro and in vivo. (A) Wound healing assay displays the cell migration after 24 h in CRC cells after differential expression of NQO1. (B) Transwell cell assay was used to detect the changes of migration ability of HCT-116 and DLD-1 cells after differential expression of NQO1. (C) Lung metastasis model was used to detect the effect of NQO1 differential expression on the metastatic ability of colorectal cancer cells in nude mice (n = 5 per group). Representative images showed the number of lung metastasis nodules, the size of the tumors. (D) HE staining of the lung tissues and IHC staining of E-cadherin and Vimentin in the four groups of xenograft tumor tissues. (E) Morphological changes of HCT-116 and DLD-1 cells after lentivirus transfection with differential expression of NQO1. (F) Western blot assay was used to detect the protein expression of EMT-related markers and transcription factors in CRC cells after differential expression of NQO1. (G) IF staining assay was used to detect the expression of epithelial marker E-cadherin and mesenchymal marker Vimentin in CRC cells after differential expression of NQO1 (blue: nuclear staining; green: E-cadherin staining; red: Vimentin staining). (H) Western blot assay detects the expression of EMT-related markers in the xenograft tumor tissues after differential expression of NQO1. **P < 0.01

Fig. 4

NQO1 Promotes anoikis resistance of CRC cells. (A-B) GO pathway analysis and GeneMANIA found that NQO1 may participate in biological processes and the correlation between NQO1 and apoptosis-related protein. (C) The effect of differential expression of NQO1 on apoptosis of CRC cells was detected by Hoechst 33,342 staining. (D) The effect of differential expression of NQO1 on apoptosis of CRC cells was detected by Annexin V-PE/7-AAD assay using flow cytometry. (E) Differential expression of NQO1 on HCT-116 and DLD-1 cells were cultured successfully and photographed under microscope. (F) Western blot determination of apoptosis markers protein expression in CRC cells after differential expression of NQO1

Wang Y., Zhou H., Liu Y., Zhao X., Wang S, & Lin Z. (2025). miR-485-5p/NQO1 axis drives colorectal cancer progression by regulating apoptosis and aerobic glycolysis. Cancer Cell International, 25, 41.

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COAD and Gastric Cell Line Culture

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Ten COAD cell lines, including “HT-29, HCT-116, SW480, SW620, DLD-1, Caco-2, LoVo, RKO, Colo205, and LS174T” and five control gastric cell lines, including “AGS, MKN-45, NCI-N87, SNU-1, and KATO III” were purchased from the ATCC, USA. These cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Scientific) media supplemented with fetal bovine serum (FBS, Thermo Scientific) and antibiotics, maintained at 37 °C in a humidified atmosphere with 5% CO₂.

Zhao Z., Feng X., Chen B., Wu Y., Wang X., Tang Z., Huang M, & Guo X. (2025). CDCA genes as prognostic and therapeutic targets in Colon adenocarcinoma. Hereditas, 162, 19.

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Cell Line Culture Protocol

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The Jurkat and SW480 cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). The OECM-1 cell line was obtained from Professor Ann-Joy Cheng (Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan). Jurkat cells and SW480 cells were cultured in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% fetal bovine serum (FBS). OECM-1 cells were cultured in RPMI medium supplemented with 5% FBS. All media were supplemented with 1% penicillin/streptomycin and were filtered using a 0.2 μm filter before use.

Ho C.R., Tsai H.J., Wang J.R., Wang C.T., Chiou C.C., Cheng J.C., Chiang S.F, & Tseng C.P. (2025). Development of PowerMag System II for Isolation of Circulating Tumor Cells with Improved Purity. Biomedicines, 13(2), 431.

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Colon Cancer Cell Line Characterization

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SW480, HCT-15, HCT-116, HT-29 (colon cancer), FHC (normal colon epithelial), and CCD-18Co (normal colon tissue) cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). The SW480sub cell line is a subclone isolated from SW480 cells in-house [12 (link)]; these cells display higher matrix adhesion capacity than SW480 cells. SW480, HCT-15, HCT-116, and HT-29 cells were maintained at 37 °C/5% CO₂ in RPMI1640 supplemented with 10% FBS. Stable cells (TMEM52B-suppressed vs. control cells) established from the HCT-15 cell lines were described previously [12 (link)]. FHC cells were maintained in DMEM/F12 supplemented with 10% FBS, 10 mM HEPES, 5 µg/mL insulin, 100 ng/mL hydrocortisone, 5 µg/mL transferrin, 10 ng/mL human recombinant EGF. CCD-18Co cells were maintained in Eagle’s MEM with 10% FBS. C8161 cells (melanoma) were a kind gift from Dr. C-R Jung (KRIBB, Korea) [13 (link)]. Cells were checked for mycoplasma and their identities were confirmed using STR-PCR analysis.

Lee Y., Ko D., Yoon J, & Kim S. (2025). TMEM52B-derived peptides inhibit generation of soluble E-cadherin and EGFR activity to suppress colon cancer growth and early metastasis. Journal of Translational Medicine, 23, 146.

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Stable Knockdown of HSF4 in Colorectal Cancer Cells

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Two human colorectal cancer cell lines, SW480 and HCT116, and a colon fibroblast cell line, CCD-18Co, were obtained from the American Type Culture Collection. SW480 cells were maintained in Leibovitz's L-15 medium (Gibco) with 10% fetal bovine serum (FBS) (Hyclone, Logan, UT, USA), 2 mM L-glutamine, 0.1 mg/mL streptomycin, and 100U/mL penicillin at 37 °C in a standard humidity incubator. HCT116 cells were cultured in RPMI 1640 medium (Invitrogen) with 10% FBS and maintained in a humidified incubator at 37 °C with 5% CO₂. CCD-18Co cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) with high glucose (Life Technology, NY, USA), supplemented with 100 mg/ml streptomycin, 100 units/ml penicillin, and 10% fetal bovine serum. The cells were maintained at 37 °C with 5% CO₂ and ≥ 90% humidity.
Genchem Biotechnology Co. Ltd. (Shanghai) provided lentiviral-based small hairpin RNA (shRNA) targeting HSF4 and a control lentivirus with scrambled shRNA. The sequences used were si-HSF4 #1: 5’-GCAAGCUGAUCCAGUGUCUTT-3', si-HSF4 #2: 5’-CGCCAACUCAACAUGUACGTT-3', and scrambled: 5’-UUCUCCGAACGUGUCACGU-3'. Experiments found that si-HSF4 #2 was more effective than si-HSF4 #1; therefore, only si-HSF4 #2 was used for shRNA constructs. SW480 and HCT116 cells were infected with these lentiviral particles and maintained in L-15 or RPMI 1640 medium containing 2 μg/mL or 1 μg/mL puromycin, respectively. After two weeks of selection, western blot analysis was performed to measure HSF4 expression, confirming the creation of stable cell lines.

Wang K., Ning S., Zhang S., Jiang M., Huang Y., Pei H., Li M, & Tan F. (2025). Extracellular matrix stiffness regulates colorectal cancer progression via HSF4. Journal of Experimental & Clinical Cancer Research : CR, 44, 30.

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Culturing and Utilizing Cancer Cell Lines

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All cancer cell lines (MC38, SW480, LoVo, HCT116) were purchased from ATCC. The cell lines were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 U/mL penicillin, and 100 µg/mL streptomycin (both Gibco) in a 5% CO₂ incubator. C57BL/6 (for experimental metastases model) were purchased from Zhejiang Academy of Medical Sciences. Female mice aged 6–8 weeks were used in the study. All animal experiments were conducted in accordance with the guidelines approved by Peking University Third Hospital (No. A2023016).

Xu N., Gao Z., Wu D., Chen H., Zhang Z., Zhang L., Wang Y., Lu X., Yao X., Liu X., Huang Y., Qiu M., Wang S., Liang J., Mao C., Zhang F., Xu H., Wang Y., Li X., Chen Z., Huang D., Shi J., Huang W., Lei F., Yang Z., Chen L., He C., Zhu H., Luo H., Gu J, & Lin J. (2025). 5‐hydroxymethylcytosine features of portal venous blood predict metachronous liver metastases of colorectal cancer and reveal phosphodiesterase 4 as a therapeutic target. Clinical and Translational Medicine, 15(2), e70189.

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Culturing Colorectal Cancer Cell Lines

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CRC cell lines Caco-2, HCT116, and SW480 (ATCC, USA) were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, and maintained at 37 °C in a humidified incubator with 5% CO₂. The immortalized normal colonic epithelial cell line FHC was cultured under the same conditions.

Li K., Dai Y.J., Zhang H, & Zhang Z. (2025). YAP1 activates SLC2A1 transcription and augments the malignant behavior of colorectal cancer cells by activating the Wnt/β-catenin signaling pathway. Cell Division, 20, 8.

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CRC Tissue and Cell Line Study

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Ten patients diagnosed with CRC at the Meizhou People's Hospital in 2024 were randomly chosen for this study. Human CRC and adjacent normal tissue samples were surgically collected from these patients. The selection criteria were as follows: (1) pathological diagnosis of CRC, (2) age 18-85 years, and (3) complete surgical resection. This study was approved by the Institutional Research Ethics Committee of Meizhou People's Hospital.
CRC cell lines HCT116, SW480, HT29, and Caco2 were purchased from ATCC (Manassas, VA, USA). The normal human colonic epithelial cell line NCM460 was obtained from Otwo Biotech (Shenzhen, China). Cells were maintained in FBS-containing Dulbecco's Modified Eagle's Medium (DMEM; Gibco, USA) under humidified conditions (37 °C, 5% CO₂).

Huang Q., Tang X., Gan C., Deng Q., Zhi S., Huang Q., Zheng X., Li X., Pan Z, & Huang M. (2025). EFHD1 Activates SIK3 to Limit Colorectal Cancer Initiation and Progression via the Hippo Pathway. Journal of Cancer, 16(4), 1348-1362.

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Culturing Colorectal Cancer Cell Lines

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CRC cell lines including HCT8, HCT116, HCT15, DLD1, SW620, SW480, RKO, MC38 and CT26 were purchased from ATCC. Cells were cultured in the DMEM/RPMI 1640 medium with 10% foetal bovine serum.

Duan X., Yeerkenbieke G., Huang S, & Feng Y. (2025). USP32 Promotes Colorectal Carcinoma Progression Through Activating NF‐κB Signalling Pathway. Journal of Cellular and Molecular Medicine, 29(6), e70457.

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