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Ethyl acetate

Manufactured by Alpha Chemika
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Ethyl acetate is a colorless, volatile liquid commonly used as a solvent in various industrial and laboratory applications. It has the chemical formula CH3COOC2H5 and a characteristic sweet, fruity odor. Ethyl acetate serves as an effective solvent for a range of organic compounds, including fats, oils, gums, and resins.

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Lab products found in correlation

Diethylene glycol, by Merck Group (2 mentions) Acetonitrile, by Alpha Chemika (2 mentions) Potassium hydroxide koh pellets, by Honeywell (2 mentions) Dimethyl sulfoxide (dmso), by Alpha Chemika (2 mentions) Triethylamine, by Loba Chemie (1 mentions) Methanol, by Alpha Chemika (1 mentions) Ethanol, by Alpha Chemika (1 mentions) Sodium hydroxide (naoh), by Loba Chemie (1 mentions) Mueller hinton agar (mha), by Loba Chemie (1 mentions) Merck silica gel 60 f254, by Merck Group (1 mentions) Waters lct premier mass spectrometer, by Waters Corporation (1 mentions) L arginine, by Thermo Fisher Scientific (1 mentions) Thymoquinone tq, by Merck Group (1 mentions) Trifluoroacetic acid tfa, by Alpha Chemika (1 mentions) Hematoxylin and eosin, by Alpha Chemika (1 mentions) Trifluoroacetic acid, by Alpha Chemika (1 mentions) L lysine, by Thermo Fisher Scientific (1 mentions)

5 protocols using ethyl acetate

1

Synthesis and Characterization of Metal Complexes

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All chemicals and regents used for this study were analytical grade and used without further purification. 1,10-phenanthroline monohydrate (BDH chemical Ltd., Poole, England), copper (II) acetate monohydrate [Cu(CO2CH3)2·H2O], triethylamine, NaHCO3, NaOH, and Mueller-Hinton agar were purchased from Loba Chemie PVT Ltd., Addis Ababa. Metformin hydrogen chloride (Met.HCl) and ciprofloxacin hydrogen chloride (Cip.HCl) were obtained from Cadila Pharmaceuticals PLC, Ethiopia. Methanol, ethanol, HCl, DMSO, ethyl acetate and dichloromethane (DCM) were purchased from Alpha Chemika, Addis Ababa, Ethiopia. Thin Layer Chromatography (MERCK Silica gel 60 F254) together with UV Cabinet (UV-Vis lamp at 254 and 365 nm) were used to monitor the progress of the chemical reactions. The melting points of the complexes were determined using capillary tubes (Thiele tube). UV-Vis spectrophotometer (SM-1600 Spectrophotometer), FTIR (Perkin-Elmer BX spectrometer, Shimadzu Corporation, Japan), TGA/DTC (DTG-60H SHIMADZU thermal analyzer) and High resolution mass spectra (Waters-LCT-Premier mass spectrometer) were used to characterize the synthesized metal complexes.
Alem M.B., Damena T., Desalegn T., Koobotse M., Eswaramoorthy R., Ngwira K.J., Ombito J.O., Zachariah M, & Demissie T.B. (2022). Cytotoxic mixed-ligand complexes of Cu(II): A combined experimental and computational study. Frontiers in Chemistry, 10, 1028957.
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2

Phytochemical Screening and Analysis

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Distilled water, absolute methanol 99.9% (Hrego Chemical Ethiopia PLC), loperamide hydrochloride (Medochemie Ltd, Cyprus), atropine sulfate (Humanwell Pharmaceutical PLC, Ethiopia), castor oil (Amman Pharmaceutical Industries, Jordan), ethyl acetate (Alpha Chemika, India), activated charcoal (SD Fine Chemicals Limited, India), Tween 80 (Atlas Chemical Industries, India) were chemicals, drugs or reagents used. Additional lab reagents and chemicals were also used in the phytochemical screening test.
Worku S.A., Tadesse S.A., Abdelwuhab M, & Asrie A.B. (2023). Antidiarrheal activities of methanolic crude extract and solvent fractions of the root of Verbascum sinaiticum Benth. (Scrophularaceae) in mice. Heliyon, 9(6), e16386.
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3

Synthesis and Characterization of Novel Thymoquinone Derivatives

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Thymoquinone (TQ) and diethylene glycol were obtained from Sigma (Pool, UK). The terephthaloyl chloride, p-toluenesulfonic acid monohydrate and L-arginine were obtained from Acros Organics (Geel, Belgium). The ethyl acetate, trifluoroacetic acid (TFA), dimethyl sulfoxide (DMSO), toluene, acetone, and acetonitrile were obtained from Alpha Chemika (Maharashtra, India). Glycerin was obtained from Labchem (Zelienople, PA, USA). Potassium hydroxide (KOH) pellets were obtained from Riedel-de Haën (Seelze, Germany). Methyl alcohol, diethyl ether, Tween-80, and HPLC-grade water were purchased from Tedia High Purity Solvents (Fairfield, CT, USA).
Dahmash E.Z., Ali D.K., Alyami H.S., AbdulKarim H., Alyami M.H, & Aodah A.H. (2022). Novel Thymoquinone Nanoparticles Using Poly(ester amide) Based on L-Arginine-Targeting Pulmonary Drug Delivery. Polymers, 14(6), 1082.
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4

Topical Formulation Development and Characterization

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Methanol absolute (Taflen industries, Ethiopia), n-hexane (Alpha Chemika, India), ethyl acetate (Alpha Chemika, India), white soft paraffin (Ethiopian pharmaceuticals manufacturing, SH.CO), wool fat (Blulux Laboratories Pvt. Ltd, India), hard paraffin (Lab tech chemicals), cecostearyl alcohol (Blulux Laboratories Pvt. Ltd, India), 2% nitrofurazone skin ointment (Shanghai General Pharmaceuticals Co., Ltd., China), ketamine hydrochloride injection USP (Neon Laboratories limited, India), diazepam injection (Gland pharm limited, India), 70% alcohol (Yilmana chemical production, Ethiopia), bee wax (Bo International, Delhi, India), hematoxylin and eosin (Alpha Chemika Maharashtra, India), Glacial Acetic Acid (Blulux laboratories Pvt., Ltd, India), Iodine (Riedel Laboratories Pvt., Ltd, Germany), sulphuric acid (Blulux Laboratories Pvt. Ltd, India), lead acetate (Guangdong Guanghua Chemicals, China), Potassium Iodide (Caliber Engineering, India), hydrochloric acid (Pentokay Laboratories Pvt., Ltd, India), Ammonia (Blulux Laboratories Pvt. Ltd, India), Ethanol (Hayman Laboratories Pvt. Ltd, England), chloroform (Blulux Laboratories Pvt. Ltd, India), ferric chloride (Fin Chem Industries, India), Wagner’s reagent (Research –Lab, Fin Chem Industries, India), and 10% formalin solution were procured from pharmacy, suppliers and all the reagents were of analytical grade.
Yiblet T.G., Tsegaw A., Ahmed N., Dagnew S.B., Tadesse T.Y, & Kifle Z.D. (2022). Evaluation of Wound Healing Activity of 80% Methanol Root Crude Extract and Solvent Fractions of Stephania abyssinica (Dill. & A. Rich.) Walp. (Menispermaceae) in Mice. Journal of Experimental Pharmacology, 14, 255-273.
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5

Synthesis and Characterization of Salmeterol Xinafoate

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FP, salmeterol xinafoate (SAL) and diethylene glycol were obtained from Sigma (Pool, UK). L-lysine and 2,5-furyl dichloride were purchased from Acros Organics (Geel, Belgium). Ethyl acetate, trifluoroacetic acid (TFA), dimethyl sulfoxide (DMSO), acetone, and acetonitrile were obtained from Alpha Chemika (Mumbai, India). Glycerin was obtained from Labchem (Zelienople, PA, USA). Additionally, potassium hydroxide (KOH) pellets were obtained from Riedel-de Haën (Seelze, Germany), while methyl alcohol, diethyl ether, and HPLC-grade water were purchased from Tedia High Purity Solvents (Fairfield, CT, USA).
Alyami M.H., Dahmash E.Z., Ali D.K., Alyami H.S., AbdulKarim H, & Alsudir S.A. (2022). Novel Fluticasone Propionate and Salmeterol Fixed-Dose Combination Nano-Encapsulated Particles Using Polyamide Based on L-Lysine. Pharmaceuticals, 15(3), 321.
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