In total 13 SKCM cell lines, including A375, SK-MEL-2, SK-MEL-5, SK-MEL-28, SK-MEL-31, SK-MEL-37, SK-MEL-119, SK-MEL-147, SK-MEL-187, SK-MEL-239, WM115, WM266-4, WM793 and 07 control skin cell lines, including HaCaT, NHDF-AD, HEK293T, HUVEC, BJ, NHEK, and HDFa were purchased from the ATCC. These cell lines were cultured under standard conditions in Dulbecco’s Modified Eagle’s Medium (DMEM) or RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and antibiotics, and maintained at 37 °C in a humidified atmosphere containing 5% CO2.
Hek293t
Manufactured by American Type Culture Collection
Sourced in United States, China, Germany, United Kingdom, France, Japan, Belgium, Israel, Canada, Switzerland, Italy, Spain, India, Sweden, Australia, Sao Tome and Principe, Austria
About the product
HEK293T is a human embryonic kidney cell line that is commonly used in cell and molecular biology research. It is a highly transfectable cell line, meaning it can efficiently incorporate exogenous DNA or RNA, making it a valuable tool for gene expression, protein production, and other applications.
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Glutamax, by Thermo Fisher Scientific (198 mentions)
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Fetal bovine serum (fbs), by Merck Group (179 mentions)
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Vero e6, by American Type Culture Collection (151 mentions)
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Market Availability & Pricing
The HEK293T/17 cell line, also known as 293T/17, is commercially available from the American Type Culture Collection (ATCC). It is an officially listed product under catalog number CRL-11268, and can be purchased directly through ATCC's website. The current price for a vial of this cell line is $555.00.
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Product FAQ
6 503 protocols using «hek293t»
1
SKCM Cell Lines and Controls Cultivation
2
Culturing Cell Lines and Primary MM Cells
HEK293T, H929, MM.1S, Jurkat, and Kasumi-1 cell lines were purchased from American Type Culture Collection. All cells were cultured in a humidified chamber at 37℃ with an atmosphere comprising 5% CO2.
Bone marrow mononuclear cells (BMMNCs) were obtained from patients with newly diagnosed MM (NDMM, n = 3), and relapsed/refractory MM (RRMM, n = 3) who visited the Department of Hematology, Jiangxi Provincial People's Hospital (Jiangxi, China). The characteristics of the patients with MM involved in this study are shown in Tables2 and 3 . Primary MM tumor cells were isolated from BMMNCs using anti-CD138 microbeads (Miltenyi Biotec, Germany) and cultured in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 15% FBS, 100 ng/mL rhFLT3-L, 100 ng/mL rhSCF and 50 ng/mL rhTPO (MCE, China).
Bone marrow mononuclear cells (BMMNCs) were obtained from patients with newly diagnosed MM (NDMM, n = 3), and relapsed/refractory MM (RRMM, n = 3) who visited the Department of Hematology, Jiangxi Provincial People's Hospital (Jiangxi, China). The characteristics of the patients with MM involved in this study are shown in Tables
3
Lung Cancer Tissue Collection and Cell Culture
Eighty-three paired lung cancer tissues and adjacent normal tissues were collected from Changchun Tumor Hospital. All pathological specimens were confirmed by experienced pathologists. Tissues were collected during surgical operations and promptly stored in liquid nitrogen. The clinical characteristics of the patients are presented in Table S1 . The study was approved by the Institutional Review Committee of Changchun Tumor Hospital, and conducted in accordance with the principles of Declaration of Helsinki. The informed consents were obtained from all participants. Human lung cancer cell lines (A549 and H1299) and the human embryonic kidney cell line HEK293T were purchased from the American Type Culture Collection (Manassas, USA). Cells were cultured in DMEM medium (Gibco, USA) containing 10% FBS at 37℃ with 5% CO2.
4
Cell Culture Conditions for Multiple Cell Lines
HEK293T (ATCC) and COS7 (ATCC) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (NEWZERUM) and 1% penicillin/streptomycin (Hyclone) at 37 °C with 5% CO2. THP-1 (ATCC) cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 (1640, Gibco) supplemented with 20% fetal bovine serum (NEWZERUM) and 1% penicillin/streptomycin (Hyclone) at 37 °C with 5% CO2. HCMEC/D3 (ATCC) cells were cultured in Endothelial Cell Medium (Thermal) supplemented with 5% fetal bovine serum (NEWZERUM) and 1% penicillin/streptomycin (Hyclone) at 37 °C with 5% CO2.
5
Hedgehog Pathway Activation in Cell Lines
NIH/3T3 (Thermo fisher), Daoy (ATCC), and HEK293T (ATCC) cells were cultured in complete media DMEM (high glucose, Biowest) supplemented with 10% fetal bovine serum(EurX), stable glutamine (Biowest), non-essential amino acids (Thermo Fisher), sodium pyruvate (Thermo Fisher), and penicillin/streptomycin solution (Thermo Fisher).
Gli3R-HA TetOn NIH/3T3 stable cell line was generated with the use of specially designed LT3GEPIR-Gli3R-HA vector and 3rd generation lentiviral system. Cells were reselected under the puromycin on every other passage.
Hedgehog pathway was induced by SAG (Biomibo, No#ALX-270-426-M001)treatment 200nM for 24 h (NIH/3T3 cells) or 500nM for 48 h (Daoy cells) in culture media containing 0.5% of fetal bovine serum. To induce Gli3R-HA expression stable cell lines were treated with doxycycline 300ug/ml for 24 h in culture media containing 0.5% of fetal bovine serum.
Gli3R-HA TetOn NIH/3T3 stable cell line was generated with the use of specially designed LT3GEPIR-Gli3R-HA vector and 3rd generation lentiviral system. Cells were reselected under the puromycin on every other passage.
Hedgehog pathway was induced by SAG (Biomibo, No#ALX-270-426-M001)treatment 200nM for 24 h (NIH/3T3 cells) or 500nM for 48 h (Daoy cells) in culture media containing 0.5% of fetal bovine serum. To induce Gli3R-HA expression stable cell lines were treated with doxycycline 300ug/ml for 24 h in culture media containing 0.5% of fetal bovine serum.
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