ChIP-IT High Sensitivity kit
The ChIP-IT High Sensitivity kit is a laboratory product developed by Active Motif for chromatin immunoprecipitation (ChIP) experiments. The kit is designed to provide high-sensitivity detection of DNA-protein interactions in small cell samples.
Lab products found in correlation
168 protocols using ChIP-IT High Sensitivity kit
Quantifying H3K27ac Enrichment in HEK293 Cells
Chromatin Preparation from Liver Tissue
Chromatin Preparation and GR Enrichment
Immunoprecipitation (IP) and DNA elution. 25µg of chromatin (using Nanodrop-measured concentration) was incubated overnight at 4°Cwith a GR antibody cocktail (Protein-Tech 24050-1-A (lot 00044414) and Cell Signaling D8H2 (lot 2) (2µl of each per IP reaction)). As described (47) , to permit direct comparison between samples, and to control for technical variation between ChIP reactions, a spike-in ChIP normalisation strategy was employed (31) (50) . Duplicates were removed with Picard (v2.18.14, Broad Institute). For published ChIP-seq and DNase-seq data, the sratoolkit package (v2.9.2, NCBI) was used to download FASTQ files from the GEO Sequence Read Archive. These were then processed as above.
ChIP-qPCR Analysis of Histone Modifications
ChIP Assay for FoxO1 Binding
ChIP-qPCR Analysis of Histone Modifications
Chromatin Immunoprecipitation of PC3 Cells
Chromatin immunoprecipitation with H3K27ac antibody
ChIP-Seq Analysis of Chromatin Modifications
Chromatin Immunoprecipitation in Microglial Cells
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