DNase I
DNase I is a type of enzyme that catalyzes the hydrolytic cleavage of DNA. It is commonly used in molecular biology and biotechnology applications.
Lab products found in correlation
89 protocols using DNase I
Isolation of Lymph Node and Skin Cells
Isolation and Phenotyping of Liver Cells
Non-parenchymal cells (NPCs) remaining in suspension were collected and centrifuged at 300 × g for 10 min at 4°C. Pelleted cells were resuspended in ice-cold-HBSS, mixed with freshly prepared 30% Histodenz (Sigma-Aldrich), and centrifuged at 1,500 × g for 20 min at 4°C. The NPCs at the Histodenz interface were collected, washed, and suspended in FACS buffer for analysis. The NPCs were phenotyped using dye-conjugated monoclonal antibodies specific for CD45 (clone 30-F11), F4/80 (clone BM8), and CD31 (clone 390) (BD Biosciences, Franklin Lake, NJ, United States). Stained samples were acquired on a multichannel cytometer BD LSR II equipped with FACS Diva software (BD Biosciences; Franklin Lake, NJ, United States) and analyzed using FlowJo 7.6.5 (Becton, Dickinson Company; Ashland, OR, United States).
Purification of Male Germ Cells
Extraction and Characterization of Chondroitin and Hyaluronic Acid
The powder obtained from the control sample was further purified to verify the production of chondroitin in S. equi subsp. zooepidemicus-pNZ8148kfoAkfoC, through structure determination by NMR analysis.
Isolation and Stimulation of Fibroblastic Stromal Cells
Lipid Mediators in Inflammatory Response
Isolation and Enrichment of Diverse Cell Populations
Isolation and Activation of Lymph Node Fibroblasts
Isolation of Podocytes from Murine Kidneys
Ubiquitination of H3 Histone Variants
For semiquantitative analysis of the GFP-H3 wt or K18A, K23A, K18A-K23A and R2A ubiquitination, the GFP fusion constructs were co-expressed with HA-ubiquitin in HEK 293T cells and 2 days after transfection, the cells were harvested as described above and further processed as reported previously29 (link).
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