Mem1x

Human dual-color variant PC3 prostate cancer cells expressing H2B-eGFP and DsRed2 were from Anticancer; luciferase-expressing PC3 cells were provided by Dr. Gary Gallick, UT MD Anderson Cancer Center. Cells were maintained in DMEM (Corning), 10% fetal calf serum (Sigma), penicillin and streptomycin (both 100 μg/ml, Sigma). Human C4–2B cells (provided by Dr. Timothy Thompson, UT MD Anderson Cancer Center) expressing H2B/mCherry and LifeAct-GFP were cultured in RPMI (Corning), 10% fetal calf serum (Sigma), penicillin and streptomycin (both 100 μg/ml, Sigma) and 1% HEPES. The identity of tumor cell lines was verified by Short Tandem Repeat DNA profiling (Characterized Cell Line Core Facility, M.D. Anderson Cancer Center). ASC52telo telomerase reverse transcriptase immortalized adipose tissue derived mesenchymal stem cells (hMSCs, ATCC) were maintained in Minimum Essential Medium (MEM1X, Corning), supplemented with 17% fetal calf serum, vitamins (Sigma), non-essential amino acids (Sigma), sodium pyruvate (Gibco), penicillin and streptomycin (both 100 μg/ml, Sigma). To induce osteoblastic differentiation, hMSCs were cultured in osteogenic medium (DMEM 1X, supplemented with 10% calf serum, penicillin and streptomycin, 50 μg/ml L-ascorbic acid, 10 mM β-glycerophosphate, 0.1 μM dexamethasone from Sigma).

Apigenin (> 98% purity) was procured from Indofine Chemical Co. Inc (Hillsborough, NJ, USA). WPI was a kind gift provided by Hilmar Ingredients (Hilmar, CA, USA). Human colon HCT-116 and HT-29 cancer cell lines were ordered from American Type Culture Collection (Manassas, VA, USA). The minimum essential medium (MEM), 1X, was from Corning Inc. (Corning, New York, NY, USA). Fetal bovine serum (FBS) was ordered from Life Technologies (Carlsbad, CA, USA). Unless stated otherwise, other chemicals were products of Thermo Fisher Scientific (Pittsburgh, PA, USA).