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Anti-rat BTLA

Manufactured by Abbiotec
Sourced in United States

Anti-rat BTLA is a laboratory reagent used to detect the expression of BTLA (B and T Lymphocyte Attenuator) in rat samples. BTLA is an immune checkpoint receptor that plays a role in regulating T cell responses. This reagent can be used in various experimental techniques, such as flow cytometry, to analyze the presence and levels of BTLA on immune cells.

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2 protocols using Anti-rat BTLA

For protein extraction, cells were lysed in RIPA buffer containing protease inhibitors (Sigma Aldrich, USA). Protein concentration was measured using the BCA Protein Assay (Thermo Fisher Scientific, Inc., USA). Equal amounts of protein samples were separated by 10% SDS-PAGE and blotted onto a polyvinylidene fluoride (PVDF) membrane (Millipore, Billerica MA, USA) for immunoblotting assays. The primary antibodies included anti-rat BTLA (Abbiotec CA, USA), NFATc1 (Santa Cruz CA, USA), NFATc2 (Santa Cruz Biotechnology, CA, USA), P-IκB (CST, Inc., USA), NF-κB P-P65 (CST, Inc., USA), NF-κB P65 (CST, Inc., USA), JNK (CST, Inc., USA), P-JNK (CST, Inc., USA), ERK1/2 (CST, Inc., USA), P-ERK 1/2 (CST, Inc., USA), p38 MAPK (CST, Inc., USA), and P-p38 MAPK (CST, Inc., USA), or GAPDH (CST, Inc., USA) at 4 °C. The chemiluminescent reaction (ECL) was used to reveal the bands, and the band intensity and volume were determined using Image Lab software (Bio-Rad CA, USA).
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The proteins of MLR cells were extracted, equal amounts of protein samples underwent SDS-PAGE by 10% gels and were blotted onto a polyvinylidene fluoride (PVDF) membrane (Billerica, MA, USA), Then, the membranes were incubated by primary antibodies included anti-rat BTLA (1:1,000, Abbiotec, CA, USA), anti-rat CTLA-4 (1:1,000, Santa Cruz CA, USA) or GAPDH (1:1,000, CST, Inc., USA) before incubation with HRP-conjugated secondary antibody (1:4,000, CST, Inc., USA). Finally, the chemiluminescent reaction was performed to reveal the bands, and the band intensity and volume were clarified by Image Lab software (Bio-Rad CA, USA).
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