Hepatic tissue was collected on d 6 and 13 of the experiment. The cow was restrained, and the areas covering the 10th to 12th right intercostal spaces were scanned by ultrasonography (Aloka SSD-500V equipped with a 3.5-MHz convex transducer, Aloka Co. Ltd., Tokyo, Japan) to determine the location for sampling hepatic tissue. The area was shaved and thoroughly disinfected with 3 alternating rounds of povidone-iodine scrubbing followed by a 70% isopropyl alcohol rinse. Upon disinfecting the surgical site, 15 mL of a solution containing 2% lidocaine hydrochloride was administered in the subcutaneous space and intercostal muscles. The surgical site was disinfected again as described previously. After a 2-cm long incision of the skin, a stainless-steel percutaneous hepatic biopsy tool (Aries Surgical, Davis, CA) was introduced aiming for the hepatic tissue visualized previously by ultrasonography and approximately 1 g of tissue was collected. The tissue was placed on filter paper, rinsed with sterile saline, sliced into 3 sections, transferred into 3 separate cryovials, snap-frozen in liquid N, and stored at -80°C until analysis.
Ssd 500v
Manufactured by Aloka
45 citations
Sourced in Japan, United States, Spain
About the product
The SSD-500V is a diagnostic ultrasound system manufactured by Aloka. It is designed for general diagnostic applications. The system features a high-resolution display and advanced imaging capabilities.
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45 protocols using «ssd 500v»
1
Bovine Hepatic Biopsy Protocol
2
Ultrasound Imaging of Carcass and Mammary Gland
Ultrasound images of the carcass and the mammary gland were taken on d 0 of the experiment and d 12 of each collection period. For the carcass characteristics, measurements of the gluteus medius and the biceps femoris muscle intercessions were taken by scanning between the 12th and 13th ribs and the rump in the P8 region. We used an 18-cm linear array ultrasound instrument (Aloka SSD-500V, Aloka Co. Ltd., Tokyo, Japan) operated at a frequency of 3.5 MHz. A standoff (Aloka long standoff guide-beef, Aloka Co. Ltd. Tokyo, Japan) and vegetable oil were used for adequate acoustic contact between the transducer, the standoff, and the animals' skin. Ultrasound images were recorded and later analyzed for backfat thickness and loin depth using the BioSoft Toolbox II for 200 Beef (Biotronics Inc., Ames, Iowa, USA) software.
Mammary gland ultrasound images were taken using a micro convex transducer (Mindray DP2200, Shenzhen, China), operating at 6 MHz (Albino et al., 2017a) . Images of each mammary quarter were taken in a standardized position, with an inclination of 45° in relation to teat insertion, and recorded in bitmap format, a technique described by Albino et al. (2017a) . Mammary gland ultrasound images were evaluated for pixel values in an 8-bit format as defined by Albino et al. (2017a) using ImageJ software (2011; National Institutes of Health, Bethesda, MD). The pixel value was estimated according to the brightness on a scale of 256 shades of gray (0 = black and 256 = white). Before the analysis, the software was calibrated for 100 pixels/cm pixel scale using the straight tracer tool. The pixel value of each mammary quarter was obtained as the mean from 3 squares (16 mm 2 each) randomly collected near the ductal structures from each image of each quarter. Subsequently, the pixel value of the whole mammary gland was obtained as an average value of each mammary quarter.
Mammary gland ultrasound images were taken using a micro convex transducer (Mindray DP2200, Shenzhen, China), operating at 6 MHz (Albino et al., 2017a) . Images of each mammary quarter were taken in a standardized position, with an inclination of 45° in relation to teat insertion, and recorded in bitmap format, a technique described by Albino et al. (2017a) . Mammary gland ultrasound images were evaluated for pixel values in an 8-bit format as defined by Albino et al. (2017a) using ImageJ software (2011; National Institutes of Health, Bethesda, MD). The pixel value was estimated according to the brightness on a scale of 256 shades of gray (0 = black and 256 = white). Before the analysis, the software was calibrated for 100 pixels/cm pixel scale using the straight tracer tool. The pixel value of each mammary quarter was obtained as the mean from 3 squares (16 mm 2 each) randomly collected near the ductal structures from each image of each quarter. Subsequently, the pixel value of the whole mammary gland was obtained as an average value of each mammary quarter.
Corresponding organizations : Universidade Federal de Viçosa, University of Guelph, Washington State University
3
Ultrasound Evaluation of Pork Carcass
The difference between the initial and final weights, divided by the 60 experimental days, was considered the daily weight gain (DWG). Dry matter intake (DMI) was obtained from the difference between the feed offered and rejected daily. Feed conversion ratio (FCR) was calculated as the ratio between daily feed consumption and DWG. At 55 d into the experimental period, the loin eye area of the Longissimus dorsi muscle and the backfat thickness were evaluated using ultrasound with a signal transducer (Model 5040, Aloka ® SSD-500 V, Tokyo, Japan). The images were processed with Centralized Ultrasound Processing software, using the transactional plane between the 12th and 13th ribs [18] (link).
Corresponding organizations : Colegio de Postgraduados, Universidad Nacional Autónoma de México
4
Postpartum Bovine Liver Biopsy
Hepatic tissue was collected once for biopsy on day (mean ± SD) 8.3 ± 1.5 (range from d 6 to 11) postpartum. Briefly, the cow was restrained, and the areas covering the 10th to 12th right intercostal spaces were scanned by ultrasonography (Aloka SSD-500V equipped with a 3.5-MHz convex transducer, Aloka Co. Ltd.) to determine the location for sampling hepatic tissue. The area was shaved and thoroughly cleansed with 3 alternating rounds of povidone-iodine scrubbing followed by a 70% isopropyl alcohol rinse. Upon disinfecting the surgical site, 15 mL of a solution containing 2% lidocaine hydrochloride was administered in the subcutaneous space and intercostal muscles. The surgical site was disinfected again as described previously. After a 2-cm incision of the skin, a stainless-steel percutaneous hepatic biopsy tool (Aries Surgical) was introduced aiming the hepatic tissue previously visualized by ultrasonography and approximately 1 g of tissue was collected. The tissue was placed in filter paper, rinsed with sterile saline, sliced into 3 sections, transferred into 3 separate cryovials, snap-frozen in liquid N 2 , and stored at -80°C until analyses.
Corresponding organizations : University of Florida
5
Genetic Factors Influencing Pork Quality
The Dingyuan pigs were raised in the Ankang Agriculture and Animal Husbandry Company, Dingyuan County, Anhui Province, with consistent feeding conditions. Over 200 unmated sows that were 10 months old and showed normal growth were selected and their body weight and live backfat thickness were measured using a platform scale and a B-ultrasound instrument (SSD-500V, ALOKA, Japan). Based on the measurements, 12 individuals with similar body weights were selected such that six had extremely high live backfat thickness and six had extremely low live backfat thickness. The pigs were slaughtered to measure the carcass weight, backfat thickness and lean meat percentage. The LD muscle and back subcutaneous fat tissues were collected, immediately frozen in liquid nitrogen, and stored at − 80 °C.
Corresponding organizations : China Agricultural University, Anhui Academy of Agricultural Sciences, Institute of Animal Sciences
Top 5 most cited protocols using «ssd 500v»
1
Timed Bovine Follicle Aspiration
Twenty-six cows were randomly selected from the physiology herd and estrous cycles synchronized by lysing the CL with two injections of PGF2α (PG; 25 mg/mL; i.m.; Lutalyse, Pfizer Animal Health, New York, NY) 14 days apart (Figure S1A ). This protocol naturally stimulates progesterone removal with CL lysis, and an ovulatory surge of LH occurs from 56 to 72 hours after the second injection of PG. This study was conducted on these cows over multiple estrous cycles and years. An epidural was given with 2% lidocaine (5 mL/cow) and follicle aspirations were conducted at 12, 18, 24, 30, or 36 hours after administration of the second injection of PG. An ultrasound system (ALOKA SSD-500 V, ALOKA Co. Ltd., Tokyo, Japan) with a 5 MHz transvaginal convex human transducer (ALOKA UST-981-5) adapted for the bovine with attached stainless steel needle guide was used to perform transvaginal, ultrasound-guided aspiration of dominant follicles with an 18-gauge needle. The follicular fluid was aspirated and separated from granulosa cells by centrifugation. Follicular fluid was stored at –80°C until further analysis.
Corresponding organizations : University of Nebraska–Lincoln, Roman L. Hruska U.S. Meat Animal Research Center
2
Equine Body Composition Ultrasound Evaluation
Mares were evaluated every 14 d for BW and BCS, and on d 0, 28, and 56 for fat thickness (FT), longissimus dorsi area (LDA), and intramuscular fat (IMF) using an ultrasound machine (Aloka SSD-500V, Aloka, Inc., Tokyo, Japan). The musculoskeletal probe was fitted with a contoured pad and corn oil was applied to the ultrasound site to maximize echogenicity and minimize image artifacts. Measurements included the FT and LDA over the intercostal space between the 12th/13th ribs and the 17th/18th ribs, and FT over the rump. All FT and LDA measurements were taken with the transducer oriented perpendicular to the horse’s spine. Estimates of IMF were determined by capturing four independent images over the 17th/18th ribs with the transducer positioned parallel to the horse’s spine. In accordance with Westervelt et al. (1976) (link), BF percentage was estimated from an equation using rump FT measurements taken 5 cm from the midline and centered between the tuber coxae and tuber ischiadicum [% BF = 4.70 × fat depth (cm) + 8.64]. All ultrasound measurements were obtained and interpreted by a single certified technician blind to treatments (Designer Genes Technologies, Inc., Harrison, AR).
Corresponding organizations : Texas A&M University, Cargill (United States)
3
Ultrasound Measurements of Beef Muscles
On d 1 and d 90, an ultrasound device was used to measure the gluteus medius and the biceps femoris muscle intercessions and the longissimus dorsi [16 (link)]. We used an 18-cm linear array ultrasound instrument (Aloka SSD-500V, Aloka Co., Ltd., Tokyo, Japan) operated at a frequency of 3.5 MHz. Standoff ultrasound images (Aloka long standoff guide-beef, Aloka Co., Ltd. Tokyo, Japan) were recorded and later analyzed for back fat thickness and rib eye area using the BioSoft Toolbox® II for 200 Beef software (Biotronics Inc., Ames, Iowa, USA).
Corresponding organizations : Universidade Federal de Viçosa, Universidade Federal Rural do Rio de Janeiro
4
Ovariectomy for Theca Cell Collection
To allow for theca cell collection and increase recovery rate of cumulus oocyte complexes, ovariectomies were conducted. Cows were randomly selected from the herd and estrous cycles synchronized with a protocol that utilizes GnRH and a controlled internal drug release device (CIDR; 1.38 g progesterone, Zoetis, Florham Park, NJ) (Fig. S1B ). Ovariectomy (n = 64) was performed approximately 36 hours after CIDR removal, which coincided with administration of PG to eliminate a functional CL. This estrous synchronization protocol allows for better synchronization of follicular waves and thus, more follicles are retrieved from cows at similar stages of follicular development.
Prior to surgery, transrectal ultrasonography (ALOKA SSD-500 V and UST-5821-7.5, ALOKA Co. Ltd., Tokyo, Japan) was performed by a single technician to evaluate the presence and size of any large follicles or CLs. Both ovaries were removed via a right flank laparotomy using aseptic techniques similar to a previously reported procedure [26] (link).
Prior to surgery, transrectal ultrasonography (ALOKA SSD-500 V and UST-5821-7.5, ALOKA Co. Ltd., Tokyo, Japan) was performed by a single technician to evaluate the presence and size of any large follicles or CLs. Both ovaries were removed via a right flank laparotomy using aseptic techniques similar to a previously reported procedure [26] (link).
Corresponding organizations : University of Nebraska–Lincoln, Roman L. Hruska U.S. Meat Animal Research Center
5
Ultrasound-based body composition monitoring
Depths of subcutaneous (USubQ) fat and Longissimus muscle at the last rib were collected weekly using a portable real-time ultrasound scanner (Aloka SSD-500v, Aloka Co., LTD., Wallingford, CT) with a 7.5 MHz (wk 1 to 7) or a 5 MHz (wk 8 onward) transducer (all from Aloka Co., LTD., Wallingford, CT). Vegetable oil was used as the ultrasound medium. Ultrasonic measurements were used to estimate changes in fat and lean content in individual pigs throughout treatment.
Corresponding organizations : Virginia Tech
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