Leaf samples (≈0.1 g dry weight) were also collected at harvest to measure the C isotopic composition (δ13C) using an elemental analyzer (EA1108; Carlo Erba Instrumentazione, Milan, Italia) coupled to an isotope ratio mass spectrometer (Delta C; Finnigan, Mat., Bremen, Germany) operating in continuous flow mode. The δ13C values were transformed to discrimination values (Δ) according to Farquhar et al. [33 (link)] (1989), where δ air = -8‰ in Vienna Pee Dee Belemnite (V-PDB):
Lci sd
The LCi-SD is a laboratory equipment used for measuring and analyzing various parameters. It is designed to provide accurate and reliable data without making any subjective interpretations or extrapolations.
Lab products found in correlation
7 protocols using lci sd
Gas Exchange and Carbon Isotope Discrimination
Leaf samples (≈0.1 g dry weight) were also collected at harvest to measure the C isotopic composition (δ13C) using an elemental analyzer (EA1108; Carlo Erba Instrumentazione, Milan, Italia) coupled to an isotope ratio mass spectrometer (Delta C; Finnigan, Mat., Bremen, Germany) operating in continuous flow mode. The δ13C values were transformed to discrimination values (Δ) according to Farquhar et al. [33 (link)] (1989), where δ air = -8‰ in Vienna Pee Dee Belemnite (V-PDB):
Photosynthetic Parameters Measurement
Measuring Photosynthetic Performance in Plants
Gas Exchange Measurements in Flowering Plants
Photosynthesis and Chlorophyll Measurement Protocol
Chlorophyll concentration was determined following Arnon [28] (link). Approx. 0.5 g of fresh leaf material was minced and extracted overnight in the dark with 80% (v/v) acetone at -4 • C. The extract was centrifuged at 10,000× g for 5 min. The absorbance of the supernatant was read at 645 nm and 633 nm in a spectrophotometer. Chlorophyll a and b were calculated as described by Arnon (1949) (link) using the formula: Chlorophyll a (mg g -1 fresh weight) = {12.7(OD 663 ) -2.69(OD 645 ) × (V/1000 × W)} Chlorophyll b (mg g -1 fresh weight) = {22.9(OD 645 ) -4.68(OD 663 ) × (V/1000 × W)} where OD was optical density at wavelength 645 or 663 nm, V was final volume, and W was the weight of the fresh leaf sample.
Physiological Attributes Measurement in Plants
Water use efficiency = Photosynthetic rate Transpirational rate .
Physiological Responses of Plants to Environmental Factors
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