The largest database of trusted experimental protocols

2 protocols using 2 2 4 4 d4 taurocholic acid

1

Comprehensive Multi-Analyte Measurements

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies for flow-cytometry were purchased from BD biosciences (San
Jose, CA). Cytokine levels were determined using 13-plex Cytometric bead arrays
(CBA) from Biolegend (San Diego, CA). Mass-spectrometry reagents were sourced
from various vendors as follows: J.T. Baker Ultra LC/MS-grade acetonitrile
(ACN), methanol (MeOH), and water were purchased from VWR International (Radnor,
PA). LC/MS Ultra-grade formic acid (Fluka) was purchased from Sigma-Aldrich
(Saint Louis, MO, USA). Internal standards 2,2,4,4-D4 cholic acid,
2,2,4,4-D4 deoxycholic acid, 2,2,4,4-D4chenodeoxycholic acid, 2,2,4,4-D4 ursodeoxycholic acid, and
2,2,4,4-D4 lithocholic acid were purchased from Cambridge Isotope
Laboratories (Andover, MA USA). 2,2,4,4-D4 taurocholic acid was
purchased from AlsaChim (France). Millipore Amicon Ultrafree PTFE membrane
centrifugal filters (0.2 μm), and Millipore Amicon Ultra 0.5 mL 3,000
MWCO centrifugal filters were purchased from Thermo Fisher Scientific (Waltham,
MA USA)
+ Open protocol
+ Expand
2

Comprehensive Multi-Analyte Measurements

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies for flow-cytometry were purchased from BD biosciences (San
Jose, CA). Cytokine levels were determined using 13-plex Cytometric bead arrays
(CBA) from Biolegend (San Diego, CA). Mass-spectrometry reagents were sourced
from various vendors as follows: J.T. Baker Ultra LC/MS-grade acetonitrile
(ACN), methanol (MeOH), and water were purchased from VWR International (Radnor,
PA). LC/MS Ultra-grade formic acid (Fluka) was purchased from Sigma-Aldrich
(Saint Louis, MO, USA). Internal standards 2,2,4,4-D4 cholic acid,
2,2,4,4-D4 deoxycholic acid, 2,2,4,4-D4chenodeoxycholic acid, 2,2,4,4-D4 ursodeoxycholic acid, and
2,2,4,4-D4 lithocholic acid were purchased from Cambridge Isotope
Laboratories (Andover, MA USA). 2,2,4,4-D4 taurocholic acid was
purchased from AlsaChim (France). Millipore Amicon Ultrafree PTFE membrane
centrifugal filters (0.2 μm), and Millipore Amicon Ultra 0.5 mL 3,000
MWCO centrifugal filters were purchased from Thermo Fisher Scientific (Waltham,
MA USA)
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!