An Elemental Scientific Lasers NWR193 laser ablation system (Kennelec Scientific, Mitcham, Victoria, Australia) coupled to an Agilent Technologies 7700 Series ICP-MS (Agilent Technologies, Mulgrave, Victoria, Australia) was used for all experiments. Laser ablation and ICP-MS conditions were optimised by ablating a NIST 612 Trace Element in Glass CRM to tune for maximum sensitivity while ensuring low oxide formation (ThO/Th<0.3%, see Table 1 ) using our standard conditions for elemental bioimaging [20 (link)]. The ICP-MS was set to monitor isotopes 146Nd, 158Gd, and 162Dy during ablation with a 15 μm spot size scanned at 60 μm s−1 with the laser set at 20 Hz and 5% power. A 300 μm × 300 μm square was ablated in each sample in approximately the same location on the consecutive tissue sections. For super resolution reconstruction (SRR) LA-ICP-MS imaging, a 15 μm spot size was scanned at 30 μm s−1 with the laser set at 20 Hz and 5% power. Raw data was then processed with an in-house MATLAB code and FIJI as per Westerhausen et al. [21 (link)].
7700 series icp ms
Manufactured by Agilent Technologies
Sourced in United States, Japan
The 7700 Series ICP-MS is a laboratory instrument designed for elemental analysis. It uses inductively coupled plasma mass spectrometry (ICP-MS) technology to detect and quantify trace elements in a wide range of sample types. The 7700 Series ICP-MS provides precise, accurate, and sensitive measurement capabilities for a variety of applications.
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Lab products found in correlation
Mass hunter workstation version, by Agilent Technologies (1 mentions)
Agilent 1260 infinity quaternary pump vl, by Agilent Technologies (1 mentions)
Bca assay, by Thermo Fisher Scientific (1 mentions)
8800 triple quadrupole icp ms, by Agilent Technologies (1 mentions)
Ultrashield 300 mhz, by Bruker (1 mentions)
Nitric acid, by Merck Group (1 mentions)
Wx ultraseries centrifuge, by Thermo Fisher Scientific (1 mentions)
F50l 24 1.5 rotor, by Thermo Fisher Scientific (1 mentions)
2414 refractive index detector, by Waters Corporation (1 mentions)
Phosphate colorimetric assay kit, by Abcam (1 mentions)
Hi 2210 ph meter, by Hanna Instruments (1 mentions)
Microwave extraction system, by Milestone (1 mentions)
Suprapur, by Merck Group (1 mentions)
1260 series hplc, by Agilent Technologies (1 mentions)
229412 centrifuge tube, by Celltreat (1 mentions)
Optima 7300 dv, by PerkinElmer (1 mentions)
Glass microfibre filter, by Cytiva (1 mentions)
1260 infinity series lc, by Agilent Technologies (1 mentions)
Zorbax rx c8, by Agilent Technologies (1 mentions)
Toc 5 wp, by Shimadzu (1 mentions)
29 protocols using 7700 series icp ms
1
Optimized LA-ICP-MS Bioimaging of Rare Earth Elements
2
Arsenic Species Separation by HPLC-ICP-MS
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For the separation of individual arsenic species, we used an Agilent Technologies 7700 series ICP-MS instrument (Agilent Technologies, Santa Clara, CA, USA), equipped with a lowflow sample introduction system and patented high matrix introduction kit. The HPLC system (Agilent Technologies) consisted of an Agilent 1260 Infinity Quaternary Pump VL, standard auto-sampler, and thermostat column compartment, equipped with Peltier cooling and heating apparatus for providing temperature stability and application flexibility. All samples were filtered with a 0.22 μm membrane before being placed into chromatographic vials (Agilent Technologies), then placed on the auto-sampler tray. A PRP X-100 anion-exchange HPLC column (5 μm, 4.6 mm×250 mm, Hamilton, Bonaduz, Switzerland) was used for arsenic species separation. The column was packed with a 55% cross-linked polystyrene divinylbenzene copolymer functionalized with quaternary ammonium anion-exchanger groups. The outlet of the column was directly connected to the sample introduction system of ICP-MS instrument. Reading data were collected by using Mass Hunter Workstation version B.01.01 (Agilent Technologies). The operating conditions for HPLC and ICP-MS are shown in Table 1 .
3
Quantitative Copper Profiling in Human Spinal Cord
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Total copper levels in human spinal cord were measured by solution inductively coupled plasma-mass spectrometry (ICP-MS) as previously described1 (link) using an Agilent 7700 Series ICP-MS. To assess biochemical partitioning of copper, TBS-soluble and TBS-insoluble fractions were analysed using a microdroplet laser ablation (LA) ICP-MS methodology16 (link). Parallel assessment of protein content via the BCA Assay (Thermo Fisher Scientific) enabled expression of copper content in total spinal cord and the TBS-soluble and -insoluble fractions relative to protein content. In situ quantitation of copper was performed using LA-ICP-MS18 (link) utilising spinal cord embedded in Optimal Cutting Temperature compound and cryo-sectioned at 30 μm in the transverse plane. Reference to a CNS matrix-matched standard46 (link) enabled expression of in situ copper relative to tissue mass. All in situ and microdroplet LA-ICP-MS analyses utilised a NewWave Research NWR213 laser ablation system coupled to an Agilent 8800 triple quadrupole ICP-MS. Data were analysed using Iolite operating under the Igor Pro 8 suite (WaveMetrics, Inc.).
4
Determination of Pseudo-Total Metal Content
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The chemical extraction for the determination of pseudo-total metal content in the solid phase and liquid extracts was performed according to the EPA method 3051A (2007) [87 ]. Moreover, 0.5 g of solid phase and 5 mL of liquid extracts were digested with the addition of the 10 mL HNO3 and HCl (3:1) in microwave unit (Milestone Microwave Extraction System, Start E). After digestion, the extract was filtered in the 25 mL flask. Metal content in the digested samples was then determined using the ICP-MS technique (Agilent Technologies 7700 Series ICP-MS, Santa Clara, CA, USA).
5
Characterization of X Solution
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All the chemicals for synthesis and characterization of X Solution were purchased from Sigma-Aldrich, with exception of Nitric acid for trace analysis, which was purchased from Merck.
All 31P-NMR spectra were obtained with a Bruker ULTRASHIELD 300 MHz. Delay time (d1) was selected at 2.000 s and the Pulse (p1) was selected at 11.00 µs. The number of scans was set to 100 scans. The inductively coupled plasma-mass spectrometry (ICP-MS) analysis was performed with an Agilent Technologies 7700 Series ICP-MS.
All 31P-NMR spectra were obtained with a Bruker ULTRASHIELD 300 MHz. Delay time (d1) was selected at 2.000 s and the Pulse (p1) was selected at 11.00 µs. The number of scans was set to 100 scans. The inductively coupled plasma-mass spectrometry (ICP-MS) analysis was performed with an Agilent Technologies 7700 Series ICP-MS.
6
Microdomain Carbonate Uranium Analysis by LA-ICP-MS
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Microdomain carbonate uranium content was determined by laser ablation (LA)-ICP-MS at the CAS Key Laboratory of Crust-Mantle Materials and Environments at USTC, using an ArF excimer ultraviolet (193 nm) system connected to an Agilent 7700 series ICP-MS. The analyses were conducted with a pulse rate of 10 Hz. The spot diameters were in the range of 16–60 μm, depending on the sizes of the calcite or dolomite grains. NIST 610 was used as the standard. To verify the validity of this method, two samples of primary marine carbonate (11CH738 and 11CH796) were analysed by both LA-ICP-MS and solution methods. The results of the LA-ICP-MS method (0.21 and 0.49 p.p.m., Fig. 3 and Supplementary Table 3 ) are comparable with those of the solution method (0.25 and 0.46 p.p.m., Supplementary Table 2 ).
7
Wastewater Adsorption Evaluation of Magnetic Adsorbents
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In order to evaluate the efficacy of these adsorbents, S1 , SM1 and SP1 were tested in a real wastewater treatment. Wastewater first of all was filtered through a paper filter, and after that through 0.2 microm membrane filter. Initial pH was 8.26. An adsorption set of experiments was conducted the sample dose 2.0 g/dm3 in 50 cm3 capped polyethylene vials containing 25 cm3 of effluent. The vials were placed inside constant orbital shaking at room temperature. The samples were collected 24 hours, filtered through paper filters, and the metal ions concentrations were quantified by Agilent Technologies 7700 Series ICP-MS.
Nitric acid 1 M was used for the regeneration of the magnetic adsorbents (1 h). After that they were dried during 3 h at 100 °C and used again.
Nitric acid 1 M was used for the regeneration of the magnetic adsorbents (1 h). After that they were dried during 3 h at 100 °C and used again.
8
Silver Nanoparticle Concentration Analysis
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Mass concentration of silver was determined with inductively coupled plasma mass spectroscopy (ICP-MS) on a 7700 series ICP-MS from Agilent Technologies (Santa Clara, CA) equipped with on-line internal standard delivery. Total silver was analyzed using m/z 107 and Y and In as internal standards. Calibration standards were prepared by dilution from a 1000 ppm silver standard from Inorganic Ventures (Christiansburg, VA). A calibration curve was verified for each analysis using dilutions from a 1 ppm silver standard from SPEX CertiPrep (Metuchen, NJ). To assess silver concentration in the nanoparticle suspensions, tubes were sonicated while an aliquot for dilution was taken out and acidified with 800 µl of concentrated nitric acid. The samples were then diluted to 10 ml with a 4% HNO3 0.5% HCl solution. For analysis of the supernatants, AgNP suspensions were subjected to centrifugation at 25,000×g for 90 min, using a WX Ultra Series centrifuge with a F50L-24 × 1.5 rotor (Thermo Scientific). Supernatants were carefully separated from pellets and silver concentration assessed.
9
Quantifying REE, Th, and Biomass in Bioreactors
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Rare earth element, Th, phosphate, glucose, pH, and biomass were quantified by previously described analytical methods (Brisson et al., 2016 (link)). Briefly, REE and Th concentrations were measured using an Agilent Technologies 7700 series ICP-MS. Phosphate concentration was measured using the BioVision Phosphate Colorimetric Assay Kit. Glucose was measured by HPLC on a Waters 2695 HPLC system with a BioRad Aminex HPX-87H carbohydrate/organic acids analysis column and a Waters 2414 refractive index detector. pH was measured using a Hanna Instruments HI 2210 pH meter. Biomass was measured as total volatile solids of filter-collected samples by drying at 105°C and subsequent ashing at 550°C as described previously (Brisson et al., 2016 (link)) based on United States Environmental Protection Agency Method 1684 (US EPA, 2001 ). REE, Th, phosphate, glucose, and pH measurements were taken for six biological replicates for each time point (0, 2, 4, and 6 days after inoculation), while biomass measurements were taken for three biological replicates at time points 2, 4, and 6 days.
10
Microwave-Assisted Solid-Phase Metal Extraction
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The chemical extraction for the determination of pseudo-total metal content was performed following the EPA method 3051A for solid phase [63 ]. For this, 0.5 g of solid phase was initially digested with the addition of 10 mL HNO3 and HCl (3:1) in the microwave unit of the Milestone Microwave Extraction System, Start E. After digestion, the extract was filtered into a 25 mL flask. The metal content in the digested samples was then determined using the ICP-MS technique (Agilent Technologies 7700 Series ICP-MS). All measurements were carried out in duplicate. The expanded uncertainty of measurement determined according to ISO 11352:2012 [64 ] was in the range of 20–30%.
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