The cell lines used in this study include human breast cancer cell lines MDA-MB-231, MCF-7, and T47D, mouse breast cancer cell lines EMT-6, and human monocytes THP-1 cell line. All cells were cultured at 37 °C with 5% CO2 in a medium supplemented with 10% Fetal Bovine Serum (FBS) (Lonsera, Uruguay) and 1% penicillin–streptomycin (PS) (SV30010, Hyclone). The EMT-6, T47D, and THP-1 cells used Roswell Park Memorial Institute (RPMI) 1640 medium. MDA-MB-231 and MCF-7 cells were cultured in the DMEM medium. Human peripheral blood mononuclear cells (PBMCs) were purchased from Hycells (Shanghai) with informed consent from the donors and cultured in RPMI 1640 medium.
For cells treated with drugs, the cells were generally incubated overnight until the cell density reached 60% and incubated for a while in a fresh medium containing the corresponding drugs. Unless otherwise specified, paclitaxel was used at a concentration of 10 nM and doxorubicin at a concentration of 10 μM.
For cells treated with drugs, the cells were generally incubated overnight until the cell density reached 60% and incubated for a while in a fresh medium containing the corresponding drugs. Unless otherwise specified, paclitaxel was used at a concentration of 10 nM and doxorubicin at a concentration of 10 μM.
Free full text: Click here
