Following an established protocol [26 (link), 27 (link)], WT BALB/c mice were immunized with three intraperitoneal injections of OVA/alum (OVA (Sigma), 50μg/100μl aluminum hydroxide (alum, Thermo Scientific)) in seven-day intervals (WT-OVA/alum model). Adjuvant-free sensitization to OVA in Was−/− mice was achieved by gavaging OVA (5mg/200μl in PBS) seven times in five day intervals. Sensitized mice were challenged by gavage with 50 mg OVA in 200 μl PBS. Anaphylaxis was measured as drop in core temperature with implantable temperature transponders (IPTT-300, Biomedic Data Systems, Seaford, Del) [28 (link)].
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OVA-Induced Anaphylaxis in Mice
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Adjuvant
Alum
Aluminum hydroxide
Anaphylaxis
Balb c mice
Gavage
Intraperitoneal injections
Mice
Ova alum
Corresponding Organization : University of Veterinary Medicine Vienna
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Variable analysis
independent variables
- Type of mice (WT BALB/c vs. Was(-/-) mice)
- Sensitization method (intraperitoneal injections of OVA/alum vs. gavage of OVA)
- Anaphylaxis, measured as drop in core temperature
- Amount of OVA used for sensitization (50μg/100μl in WT-OVA/alum model, 5mg/200μl in Was(-/-) model)
- Route of OVA challenge (gavage with 50 mg OVA in 200 μl PBS for both models)
- WT BALB/c mice immunized with OVA/alum (WT-OVA/alum model)
- Was(-/-) mice sensitized with OVA without adjuvant
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