Two-week-old seedlings of hydroponically-grown rice were exposed to a nutrient solution with either 0, 0.5 or 1 μM CdCl2 for 14 days. Then the samples were washed with distilled water three times and separated into shoots and roots. Field-grown rice plants were harvested after grain ripening and separated into straw (shoot) and brown rice (grain).
All samples were dried at 70 °C for 3 d, and then digested with an acid mixture of HNO3:HClO4 (6:1 [v/v]) as described previously (Tang et al. 2017 (link)). The metal concentrations in the digest solutions were determined by inductively coupled plasma mass spectrometry (ICP-MS) (Agilent 7700 series, USA). The root-to-shoot translocation rates of metals were calculated as the amount of metal accumulated in the shoots as a percentage of the total amount of metal accumulated in the whole plant, as described by Miyadate et al. (2011 (link)).
All samples were dried at 70 °C for 3 d, and then digested with an acid mixture of HNO3:HClO4 (6:1 [v/v]) as described previously (Tang et al. 2017 (link)). The metal concentrations in the digest solutions were determined by inductively coupled plasma mass spectrometry (ICP-MS) (Agilent 7700 series, USA). The root-to-shoot translocation rates of metals were calculated as the amount of metal accumulated in the shoots as a percentage of the total amount of metal accumulated in the whole plant, as described by Miyadate et al. (2011 (link)).
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