Engineered Microbubbles for Biomedical Applications

Microbubbles comprised of a 90 mol% 1,2-distearoyl-sn-glycero-3- phosphocholine (DSPC) and 10 mol% 1,2-distearoyl-sn-glycero-3- phosphoethanolamine-N-[methoxy(polyethylene glycol)2000] (DSPE-PEG2000) (Avanti Polar Lipids, Alabaster, AL, USA) lipid-shell and a perfluorobutane (FluoroMed, Round Rock, TX, USA) gas-core were manufactured in-house. Size-selected microbubbles with a median diameter of 4–5 µm were isolated from a poly-dispersed microbubble distribution using a differential centrifugation method [29] (link). Their size distributions and concentrations were determined by a particle counter (Multisizer III, Beckman Coulter Inc., Opa Locka, FL, USA). Before each injection into the mouse, their concentrations were diluted using sterile saline to a final concentration of approximately 8×10⁸ number of microbubbles per mL.

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Chen H., Chen C.C., Acosta C., Wu S.Y., Sun T, & Konofagou E.E. (2014). A New Brain Drug Delivery Strategy: Focused Ultrasound-Enhanced Intranasal Drug Delivery. PLoS ONE, 9(10), e108880.

Publication 2014

DSPE-PEG2000 Multisizer III

Alabaster Centrifugation Distearoyl sn glycero 3 phosphocholine Dspe peg2000 Lipid Methoxy polyethylene glycol Microbubbles Mouse Perfluorobutane Phosphoethanolamine Poly a Saline Sterile

Corresponding Organization : Columbia University

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Variable analysis

independent variables

Lipid-shell composition (90 mol% DSPC and 10 mol% DSPE-PEG2000)
Gas-core (perfluorobutane)

dependent variables

Size distribution of size-selected microbubbles
Concentration of size-selected microbubbles

control variables

Size-selection of microbubbles using differential centrifugation
Dilution of microbubble concentration in sterile saline before injection

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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